5%唑啉草酯·双氟磺草胺可分散油悬浮剂高效液相色谱分析

Analysis of Pinoxaden+Florasulam 5% OD by HPLC

采用高效液相色谱,以乙腈+0.05%磷酸水溶液为流动相,使用C18反相柱和紫外可变波长检测器,在260 nm波长下对5%唑啉草酯·双氟磺草胺可分散油悬浮剂中有效成分进行分离和定量分析。结果表明,唑啉草酯、双氟磺草胺的线性相关系数分别为0.999 2和0.999 3,标准偏差分别为0.09和0.02,变异系数分别为2.05%和2.44%,平均回收率分别为99.28%和99.69%。

A method for separation and quantitative analysis ofpinoxaden ＋ florasulam 5% OD by HPLC was described, using acetonitrile and phosphoric acid solution （0.05%） as mobile phase, on ZORBAX SB-CI8 column and UV-absorbance detector, at 260 nm wavelength. The results showed that the linear correlation coefficients of pinoxaden and florasulam were 0.999 2 and 0.999 3, the standard deviations were 0.09 and 0.02, the variation coefficients were 2.05% and 2.44%, and the average recoveries were 99.28% and 99.69%, respectively.