罗丹明6G恒波长同步荧光猝灭法测定痕量亚硝酸根

Determination of Trace Nitrite by Constant-wavelength Synchronous Fluorescent Quenching Method

建立了恒波长同步荧光猝灭测定微量亚硝酸根的新方法。研究表明:在HCl介质中,Δλ=25nm,亚硝酸根对溴酸根氧化罗丹明6G具有催化作用,使罗丹明6G的同步荧光发生猝灭,且猝灭程度与亚硝酸根量形成线性关系。方法的检出限为0.68μg/L,线性范围为2~36μg/L。该方法可用于火腿肠中亚硝酸根含量的测定,能得到满意结果。

The constant-wavelength synchronous fluorescent quenching method is established for determination of trace nitrite. In HC1 media （△λ=25 nm）, constant-wavelength synchronous fluorescence of rhodamine 6G can be quenched because nitrite is used as catalyst for the oxidation of rhodamine 6G with bromate ion, and the linear relationship is developed between the extent of quenching and the content of nitrite. The detection limit for nitrite is 0.68μg/L with the linear range of 2-36 μg/L. The method can be used to determine trace NO2- in sausage with satisfactory results.