6个中国小型猪种SLA-DQB基因外显子2多态性分析

Genetic Polymorphism of Exon 2 of SLA-DQB Genes in Six Miniature Pig Breeds

采用PCR-RFLP法对甘孜藏猪、合作猪、滇南小耳猪、荷包猪、五指山猪和巴马小型猪SLA-DQB基因外显子2多态性进行分析。结果表明:经RsaⅠ酶切检测到5种等位基因,14种RFLP带型,遗传杂合度(He)在0.538~0.729,多态信息含量(PIC)在0.488~0.728,该位点表现为高度多态;χ~2适合性检验显示,甘孜藏猪、巴马小型猪和五指山猪3个群体处于Hardy-Weinberg平衡状态。经HinfⅠ酶切检测到3种等位基因,6种RFLP带型,He在0.315~0.524,PIC在0.291~0.461,在该位点表现为中度多态。χ~2适合性检验显示,甘孜藏猪、滇南小耳猪、巴马小型猪、荷包猪4个群体处于Hardy-Weinberg平衡状态。因此,6个小型猪种的SLA-DQB基因外显子2多态性丰富且存在明显差异。

The exon 2 polymorphism of SLA-DQB gene in Ganzhi Zang pigs（Tibet pigs）, Hezuo pigs, Diannan Small- eared pigs, Hebao pigs, Wuzhishan pigs and Bama miniature pigs were studied using PCR-RFLP technique. The results showed that there were five alleles and fourteen RFLP genotypes with restriction enzyme RsaI digestion, polymorphism of the DQB exon 2 was abundant. Its genetic heterozygosity was 0.538-0.729, and polymorphic information content was 0.488-0.728. The results ofz2 showed that five alleles ofRsa I locus in Ganzhi Zang pigs,Bama pigs and Wuzhishan pigs fit with Hardy-Weinberg equilibrium. There were three alleles and six RFLP with restriction enzyme Hinf I digestion. Its genetic heterozygosity was 0.315-0.524, and polymorphic information content was 0.291-0.461.The locus was moderately polymorphic. The results ofz2 showed that 3 alleles of HinfI locus in Ganzhi Zang pigs, Diannan pigs, Bama pigs and Hebao pigs were fit with Hardy-Weinberg equilibrium. The results suggested that there was significant difference in six miniature pigs.