不同浓度的鱼精蛋白对猪精液17℃液态保存效果的影响

Effects of Different Concentrations of Protamine on Quality of Boar Semen Stored at 17℃

鱼精蛋白具有一定的抗菌效果和保护精子的作用,为探讨不同浓度的鱼精蛋白对猪精液17℃保存效果的影响,本实验在Modena稀释液中分别添加0、5、10、20μg/m L的鱼精蛋白,在常温保存过程中,检测精子活率、路径速度、直线速度、畸形率等质量参数。结果表明:精液在17℃保存过程中,在Modena稀释液中添加10μg/m L的鱼精蛋白,精子活率、路径速度、直线速度显著高于其他组(P<0.05),畸形率显著低于其他组(P<0.05)。综上可知,17℃保存的过程中,在Modena稀释液中添加10μg/m L的鱼精蛋白能够提高精液的保存效果,延长猪精液的保存时间。

The aim of this study was to investigate that the effects of protamine on the quality of boar semen stored at 17℃. Boar semen was diluted with Modena diluent containing different concentrations （0, 5, 10, 20 μg/mL） of protamine. The sperm motility, path velocity, linear velocity, and sperm deformity rate were evaluated to analyze the effects of protamine on boar semen quality during liquid preservation at 17℃. The results showed that the process of storage at 17℃, detecting sperm motility, path velocity, linear velocity of the group on the basis of the diluent containing 10μg/mL protamine were significantly higher than that of the other groups （P〈0.05）, and sperm deformity rate of group containing 10μg/mL protamine was the lowest in all groups. In this study, our results confirmed that 10μg/mL protamine can improve the quality of boar semen during liquid storage at 17℃.