4℃冷藏保存血小板的代谢变化

Metabolic changes in platelets stored at 4℃

目的对比分析4℃冷藏保存血小板与22℃振荡保存血小板代谢情况,为制定冷藏保存血小板技术提供实验室依据。方法对4℃冷藏保存血小板于保存到d1、d3、d5、d7、d10、d14、d21,7个时间点和22℃振荡保存血小板保存到d1、d3、d5,3个时间点时采集血样,检测不同保存条件下的血小板血气指标、生物化学指标和低渗休克反应率。结果 4℃冷藏保存血小板在保存7 d内pH、Na^+、Ca^(2+)、Cl^-值无明显变化(P>0.05),22℃振荡保存血小板pH、Na^+、Ca^(2+)、Cl^-、PCO_2、PO_2、Lac、HCO_3^-、ctCO_2、Gap(K^+)保存5 d内均有明显变化(P<0.05)。血小板保存d5时,22℃保存血小板的pH值明显低于4℃保存血小板,而LDH和Lac值明显高于4℃保存(P<0.05)。LDH、pH、K^+、PCO_2、PO_2、Lac、HCO_3^-、ctCO_2、Gap(K^+)2组间比较均有差异(P<0.05)。4℃冷藏保存5 d内血小板低渗休克恢复率变化不明显(P>0.05),而22℃保存血小板HSR恢复率d5与d1相比降低53.77%。结论 4℃冷藏保存血小板与22℃振荡保存血小板在代谢方面比较,冷藏保存血小板具有代谢慢、乳酸产生少、pH降低慢及葡萄糖消耗低等特点,4℃冷藏保存血小板10-14 d时仍有一定的HSR恢复率。就血小板代谢数据比较,4℃冷藏保存血小板建议保存10-14 d。

Objective To compare the metabolic changes in platelets stored at 4℃ and those with agitation at 22℃ in order to provide an experimental basis for the preservation technology of platelets.Methods Blood samples were collected for platelets stored at 4℃ for 1,3,5,7,10,14,and 21 days as well as for those stored with agitation at 22℃ for 1,3,and5 days.The levels of gas indicators,biochemical indicators,and hypotonic shock response（HSR） were measured for platelets stored under different conditions.Results There were no significant changes in the pH,Na ^＋,Ca,and Cl-levels of platelets stored at 4℃ for 7 days（P〉0.05）.There were significant changes in the pH,Na^＋,Ca^＋,Cl^-,pCO2,pO2,lactate（Lac）,HCO3^-,ctCO2,and Gap（K^＋） levels of platelets stored with agitation at 22℃ for 5 days（P〈0.005）.On the 5th day,platelets stored at 22℃ showed significantly lower pH values than platelets stored at 4℃,while the lactate dehydrogenase（LDH） and Lac levels were significantly higher than for platelets stored at 4℃（P〈0.005）.The LDH,pH,K^＋,pCO2,pO2,Lac,HCO3^-,ctCO2,and Gap（K^＋） levels between the two groups showed significant differences（P〈0.05）.Platelets stored at 4℃ for 5 days showed no significant changes in the recovery from HSR（P〉0.05）,while those stored at 22℃ showed a reduced rate of HSR recovery of 53.77% between the 5th and 1st day.Conclusion The comparison of platelet metabolism between platelets stored at 4℃ and those agitated at 22℃ reveals that platelets stored under cold conditions show low metabolism,low lactate production,low reduction in pH,and low glucose consumption.Platelets stored at 4℃ for 10-14 days still show some HSR recovery.In terms of metabolic data,platelets stored at 4℃ are recommended to be stored for 10-14 days.