广州地区伊朗献血人群CD36表型筛查及基因型鉴定

The CD36 expression screening and genotype detection in Iranian blood donors in Guangzhou,China

目的了解广州地区伊朗籍献血者CD36表型及基因型。方法对2016年在广州血液中心捐献机采血小板的53例伊朗人献血者标本,应用流式细胞仪分别检测血小板和单核细胞上CD36抗原的表达。对CD36抗原表达异常者提取DNA,采用PCR-SBT测序做CD36的基因型鉴定并做基因突变分析。结果本组伊朗人群的CD36抗原表达异常率7.55%(4/53),其中2例的CD36抗原在血小板上表达含量低,1例为血小板和单核细胞上均无表达(即CD36Ⅰ型缺失),1例为单核细胞上CD36表达量低,由此得出CD36缺失表达的频率为1.89%(1/53)。PCRSBT法分析:CD36抗原表达异常4例的CD36基因型中,血小板上低表达CD36抗原的2例发生基因突变,分别是exon 10-975T/G和exon11 del CTA,且均为新的基因突变;2例CD36基因型正常。结论本组伊朗献血人群里CD36抗原表达异常者中存在新的基因突变,其对CD36抗原表达的影响尚需进一步认证。

Objective To learn about the phenotype and genotype of CD36 in blood donors among Iranians in Guangzhou district.Methods 53 Iranian blood donors in Guangzhou were enrolled in 2016.The CD36 expression both on platelets and monocytes was detected by flow cytometry.The DNA was extracted from the samples with abnormal CD36 expression,and its genotype was detected by PCR-SBT,the gene mutation was analyzed further.Results The frequency of abnormal CD36 expression In the 53 Iranian blood donors is 7.55%（4/53）,including 2 with low CD36 expression on platelets,1 with CD36 deficiency expression both on platelets and monocytes（type Ⅰdeficiency）,1 with low CD36 expression on monocytes.So the frequency of CD36 deficiency was 1.89%（1/53）.In the results of PCR-SBT for the 4 samples,2 samples were CD36 gene mutations,exon 10-975T/G and exon11 del CTA respectively（new mutations both）,the other 2 ones were normal CD36 genotypes.Conclusions The new 2 gene mutations have been found,and their efficiencies on expression of CD36 antigen need to be confirmed further.