膜联蛋白A1的表达对口腔鳞癌TPF化疗效果的影响

Effect of Annexin A1 expression on chemoagents of docetaxel, cisplatin, and 5-fluorouracil for oral squamous cell carcinoma

目的 :探讨在口腔鳞癌中,膜联蛋白A1的表达水平对TPF(T=多西他赛,P=顺铂,F=5氟尿嘧啶)化疗药物杀伤癌细胞疗效的影响。方法:构建Annexin A1 si RNA的反转录病毒载体,对口腔鳞癌细胞系进行干预,通过细胞增殖实验、细胞毒性实验和免疫印迹方法,分析Annexin A1表达干预对TPF化疗药物作用的影响,以及PI3K/AKT相关信号通路和细胞凋亡的影响。采用SPSS18.0软件包对数据进行双因素方差分析。结果:下调Annexin A1的表达,口腔鳞癌细胞的增殖能力增强。通过PI3K/AKT途径抑制P27kip1的表达,口腔鳞癌细胞对TPF化疗药物的敏感性提高,促进了癌细胞的凋亡。结论:Annexin A1低表达能增强口腔鳞癌细胞的增殖能力,提高对TPF的敏感性,促进癌细胞凋亡。

PURPOSE： To investigate the effect of Annexin A1 expression on chemoagents of docetacel, cisplatin, and 5- fluorouracil （TPF） in oral squamous cell carcinoma. METHODS： Annexin A1 siRNA retroviral vector was constructed and transfected into oral squamous cell carcinoma cell lines to observe the effect of Annexin A1 siRNA on cellular proliferation and cellular cytotoxieity of chemoagents of TPF. Western blot was used to analyze the protein expression when the cells treated with Annexin A1 intervention and chemoagents, especially on PI3K/AKT associated signal pathway as well as cellular apoptosis. SPSS 18.0 software package was used for two-way analysis of variance. RESULTS： Downregulation of Annexin A1 enhanced the ability of cellular proliferation, inhibited the P27kip1 expression through PI3K/AKT signal pathway, increased the sensitivity of cytotoxicity to chemoagents of TPF, and promoted cell apoptosis in oral squamous cell carcinoma cell line. CONCLUSIONS： Decreased Annexin A1 expression promotes cell proliferation, sensitivity to chemoagents of TPF, and cell apoptosis in oral squamous cell carcinoma.