脉冲电场对蛋白质与金属离子螯合作用的影响

Study on chelating of metal ion and protein in pulsed electric field

采用拉曼光谱研究脉冲电场对卵清蛋白与金属离子(Cu^(2+)、Ba^(2+)、Mn^(2+)或Ca^(2+))螯合作用的影响。试验结果表明:(1)PEF处理时间和金属离子种类都能影响金属离子和蛋白质的螯合作用,随着脉冲时间的延长,金属离子与卵清蛋白的螯合作用增强,PEF处理时间为1 695μs时,Mn^(2+)和Cu^(2+)与蛋白质的鳌合开始减弱;(2)蛋白质分子和金属离子在1 200~1 700cm^(-1)位置出现不同程度的螯合:与Mn^(2+)鳌合最强,Cu^(2+)和Ca^(2+)较弱,Ba^(2+)最弱。

The effects of pulsed electric field on chelating of ovalbumin and metal ions（Cu^2＋,Ba^2＋,Mn^2＋or Ca^2＋）and the effect of pulsed electric field on the chelating effect of ovalbumin were studied by Raman spectroscopy.Results：（1） PEF treatment time and metal ion species could affect the chelation of metal ions and protein,and the chelation of metal ions with ovalbumin was enhanced with the prolongation of pulse treatment time,when PEF treatment time was 1 695μs,the chelation of Mn^2＋and Cu^2＋with protein began to weaken;（2） The protein and metal ions chelated to different degrees at 1 200～1 700cm^-1,the strongest was Mn^2＋,followed by Cu^2＋and Ca^2＋,and the weakest was Ba^2＋.