大孔树脂纯化茶皂素及其产品性质研究

Purification of tea saponin with macroporous resin and properties of the product

研究了利用大孔吸附树脂纯化茶皂素的方法和纯化前后茶皂素性质的变化。通过8种不同极性的大孔树脂对茶皂素的吸附和解吸规律的研究,发现XR910X对茶皂素的纯化效果最好。动力学研究表明,XR910X对茶皂素的吸附符合Freundlich模型。XR910X对茶皂素纯化的最佳工艺条件为上样:样品pH 6.0,流速1.71BV/h,体积1BV;洗脱部分杂质:0.05g/100 mL NaOH溶液,流速1.71 BV/h,体积2BV;解吸:90%乙醇溶液,流速1.71BV/h,体积2BV。在上述优化的条件下,茶皂素的回收率为70.34%,产品纯度为94.26%。对比纯化前后茶皂素的相关性质,发现纯化后的茶皂素在表面活性、抗氧化活性及抑菌性能方面均比纯化前的有明显提高。

Purification of tea saponin with macroporous resin and the changes of properties of the product were investigated.With comparisons of eight kinds of macroporous resin with different polarity to the crude tea saponin through static adsorption and dynamic adsorption,XR910 Xresin was selected to refine the tea saponin.Moreover,it was found that the tea saponin adsorption by XR910 X would be matched by Freundlich Model.The optimal purification conditions were as following：the loading sample was crude tea saponin solution with pH 6.0,and the loading volume 1 BV,loading velocity1.71BV/h.Then 0.05g/100 mL NaOH solution was used to remove impurity with 1.71BV/h velocity and 2BV volume.At last,90%ethanol of 2BV volume was used as eluent to desorb the tea saponin with the ethanol flow rate 1.71BV/h.Under that condition,the recovery rate of tea saponin was 70.34%and the purity was 94.26%.In addition,the properties of tea saponin after purification in face activity and antioxidant capacity as well as antibacterial activities were much higher than those before purification.