细胞自噬与老年人带状疱疹后神经痛关系的研究

Relation of autophagy and postherpetic neuralgia in older adults

目的通过研究老年带状疱疹后神经痛与细胞自噬水平的关系，为老年带状疱疹后神经痛的防治提供新的思路。方法收取135例老年带状疱疹患者血标本，其中83例患者发展为带状疱疹后神经痛，52例患者未发展为带状疱疹后神经痛。通过吖啶橙染色，免疫印迹（Western blot）检测自噬标记蛋白LC3和自噬相关蛋白Beclin-1，检测细胞自噬水平。结果吖啶橙染色后流式细胞检测显示发展为带状疱疹后神经痛患者的平均荧光强度为0．775±0．068，未发展为带状疱疹后神经痛患者的平均荧光强度为0．342±0．025，未发展为带状疱疹后神经痛患者的细胞自噬水平显著低于发展为带状疱疹后神经痛患者，两组间差异有统计学意义（P〈0．05）。Western blot检测自噬标记蛋白LC3和自噬相关蛋白Beclin-1也均显示未发展为带状疱疹后神经痛患者的细胞自噬水平显著低于发展为带状疱疹后神经痛患者。结论高水平的细胞自噬是导致老年带状疱疹后神经痛的危险因素。

Objective To investigate the relation of autophagy and postherpetic neuralgia in elderly patients, and provide new methods for prevention and treatment of postherpetie neuralgia. Methods One hundred thirty-five specimens were obtained from elderly patients with herpes zoster, containing 83 patients with herpes zoster plus neuralgia and 52 cases with non-neuralgia herpes zoster. The level of autophagy was determined by acridine orange-stain. The protein expressions of autophagy marker protein LC3 and autophagy related protein Beclinl were detected by Western blot. Results Flow cytometer detection after acridine orange-stain showed that mean fluorescence intensity （MFI） of herpes zoster plus neuralgia patients was 0. 775 ± 0. 068 and non-neuralgia herpes zoster patients was 0. 342±0. 025. The level of autophagy of non-neuralgia herpes zoster patients was significant lower than herpes zoster plus neuralgia patients （P〈0.05）. The expressions of autophagy marker protein LC3 and autophagy related protein Beclinl detected by Western blot also showed the level of autophagy of non-neuralgia herpes zoster patients was decreased compared with herpes zoster plus neuralgia patients. Conclusions High level autophagy is risk factor for postherpetic neuralgia in elderly patients.