荷丹片对代谢综合征大鼠血脂肪细胞因子与炎症因子表达的影响

Effect of Hedan tablet on expression of serum adipocytokines and inflammatory factors in rats with metabolic syndrome

目的研究荷丹片对代谢综合征大鼠血脂肪细胞因子和炎症因子的影响。方法制备代谢综合征大鼠模型，选取造模成功的Wistar大鼠24只，随机分为正常对照组、模型组和药物治疗组各8只。正常对照组给予普通饲料喂养，模型组给予高脂高盐饲料喂养，药物治疗组给予高脂高盐饲料加荷丹片6．0g．kg^-2．d^-1灌胃给药3周。测量基线和3周干预后各组大鼠的体重、血压、血糖、血脂、血清瘦素、内脂素、脂联素及C反应蛋白（CRP）水平。结果与对照组相比，模型组大鼠体重、总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白胆固醇（LDL-C）、血糖、血清度素[（5．5±0．1）mmol／L比（5．2±0．0）mmol／L]、内脂素[（17．5±0．5）mmol／L比（16．6±0．0）mmol／L]及CRP[（1676．6±74．8）mmol／L比（1642．3±52．2）mmol／L]水平明显升高（P〈0．05），脂联素水平明显降低[（74．6±2．4）mmol／L比（81．3±2．1）mmol／L]（P〈0．05）。药物治疗组23周龄与20周龄比较，体重、血TC、TG、LDL-C、瘦素[（5．4±0．1）mmol／L比（5．9±0．2）mmol／L）]、内脂素[（16．7±0．4）mmol／L比（17．7±0．6）mmol／L）]及CRP[（1498．8±51．0）mmol／L比（1682．1±76．6）mmol／L）]水平降低（P〈0．05），血脂联素[（81．2±3．0）mmol／L比（73．9±3．1）mmol／L]水平升高（P〈0．05），而模型组没有显著变化。结论荷丹片改善代谢综合征大鼠的体重和血脂异常，可能与其调控血脂肪细胞因子及炎症因子表达水平有关。

Objective To investigate the effects of inflammatory factors in rats with metabolic syndrome Hedan tablet on serum adipoeytokines and Methods The 24 rat model of metabolic syndrome were randomly divided into three groups（n= 8, each） ：normal group were given normal diet, model group were given high-lipid and high-salt diet and intervention group were given high-lipid and high-salt diet plus Hedan tablet 6.0 g · kg^-1 · d^-1 by intragastric administration for 3 weeks. Body weight,blood pressure, blood levels of glucose, lipid, leptin, visfatin, adiponectin and CRP were measured in all rats at the beginning and the end of the study. Results Compared with normal group, model group （ model versus normal） showed significantly increased serum levels （P 〈 0.05 ） of weight, total cholesterol （ TC）, triglyceride （ TG）, low-density lipoprotein cholesterol （ LDL-C）, glucose, leptin（5.5±0.1 vs. 5.2±0.0 mmol/L）,visfatin（17.5±0.5 vs. 16.6±0.0 mmol/L and CRP（1 676.6±74. 8 vs. 1 642.3± 52.2 mmol/L）, and showed significantly decreased （P 〈 0.05 ） serum level of adiponectin（74.6±2.4 vs. 81.3±2.1 mmol/L）. Compared with 20 weeks of age group,the 23 weeks of age group（23 weeks versus 20 weeks） showed significantly decreased levels of weight, TC, TG, LDL- C,serum leptin（5.4±0.1 mmol/L vs. 5.9±0.2 mmol/L） ,visfatin（16.7±0.4 mmol/L vs. 17.7±0.6 mmol/L） and CRP（ 1 498.8 ± 51.0 mmol/L vs. 1 682. 1 ± 76.6 mmol/L （ all P〈 0.05 ）, and showed significantly increased serum adiponeetin level（81.2±3.0 mmol/L vs. 73.9±3.1 mmol/L）（P〈0.05） . But there was no significant change in model group between 20 weeks and 23 weeks of age. Conclusions Hedan tablet reduces the body weight and ameliorates blood lipid level in rats with metabolic syndrome. These effects may be related to regulation of Hedan tablet on adipocytokines and inflammatory factors.