摘要
目的考察灵芝孢子油对慢性轻度不可预见刺激模型小鼠行为学影响及可能的神经生理机制。方法采用13种不同慢性轻度不可预见刺激对雄性BALB/C小鼠连续刺激建立小鼠抑郁模型。从第3周开始连续灌胃给予3个剂量的灵芝孢子油(850、283、141.5 mg·kg^(-1)),盐酸氟西汀(10 mg·kg^(-1))或空白溶媒(植物油)2周后,测定各组小鼠行为学变化,同时采用反相高效液相色谱法(RP-HPLC)测定海马谷氨酸(GLU)和γ-氨基丁酸(GABA),酶联免疫法测定脑源性神经营养因子(BDNF)的含量。结果与模型组比较,灵芝孢子油能增加抑郁小鼠的体重、糖水偏好率和旷场实验得分(P<0.01),缩短悬尾实验和强迫游泳实验的不动时间(P<0.05,P<0.01),明显降低海马GLU的含量(P<0.01),增加BDNF含量(P<0.01),但对GABA含量无影响(P>0.05)。结论灵芝孢子油对抑郁模型小鼠表现出明显的抗抑郁作用,其抗抑郁作用可能与其降低GLU和增加BDNF相关。
OBJECTIVE To study the effects of ganoderma spore oil(GSO) on behavior of the mice with chronic unpredicta- ble mild stress (CUMS) and its possible neurophysiology mechanisms. METHODS Thirteen different kinds of chronic unpre- dictable mild stress were given to the male BALB/C mice for establishing the mouse model of depression. The mice were treated with GSO at3 doses (850, 283, 141.5 mg. kg-1 d-1) or vehicle [(oil) orfluoxetine (10 mg kg-1 d-1)] by oral ad- ministration from the 3rd week. After 2 weeks administration, the mice was evaluated by behavioral tests, and the contents of hip- pocampal glutamate (GLU) and γ-amino butyric acid (GABA) were analyzed by reversed phase high performance liquid chroma- tography (RP-HPLC). The contents of hippocampal brain derived neurotrophic factor (BDNF) were measured by ELISA kit. RESULTS Compared with model group, GSO increased the body weight, sucrose preference rate and open field test score, shortened the immobility time in the tails suspension test and forced swimming test in the depression mice (P 〈 0.05 or P 〈0.01 ). Meanwhile, GSO significantly decreased the contents of GLU (P 〈 0.01 ) and increased the contents of BDNF (P 〈 0.01 ) , and the contents of GABA did not changes (P 〉 0.05) in the hippocampus. CONCLUSION GSO shows obvi- ous anti-depressant effect on depressant model mice. The antidepressant effect of GSO may be related to decreasing GLU contents and increasing BDNF contents.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2017年第15期1325-1330,共6页
Chinese Pharmaceutical Journal
基金
浙江省省级公益性技术应用研究计划项目资助(2014C37020)
关键词
灵芝孢子油
慢性轻度不可预见刺激
谷氨酸
Γ-氨基丁酸
脑源性神经营养因子
ganoderma spore oil
chronic unpredictable mild stress
glutamic acid
γ-aminobutyric acid
brain derived neurotro- phic factor