曲古菌素A联合全反式维甲酸诱导卵巢癌SK0V-3细胞株凋亡的研究

Trichostatin A Combined with All-trans Retinoic Acid Induce Apoptosis in Human Ovarian Cancer Cell Line SKOV-3

目的探讨TSA联合ATRA对卵巢癌SKOV-3细胞的协同增殖抑制和凋亡作用。方法 TSA联合ATRA处理卵巢癌细胞SKOV-3细胞株,分别检测SK0V-3细胞增殖抑制情况和细胞凋亡率及相关蛋白表达变化。结果 TSA联合ATRA协同抑制SKOV-3细胞生长,差异有统计学意义(P<0.05);联合组SKOV-3细胞凋亡率明显升高,与对照组和单独用药组比较,差异有统计学意义(P<0.05)。结论 TSA联合ATRA在体外对卵巢癌SKOV-3细胞具有较强的协同增殖抑制和促进凋亡作用。

Objective To investigate the apoptotic effect on human ovarian cancer cells by both Trichostatin A（TSA）and all-trans retinoic acid（ATRA）and synergistic effect. Methods SKOV-3 cells were treated by different group of TSA and ATRA. The inhibitory rate of proliferation and the apoptosis rate were examined. Western blot was introduced to evaluate the protein expression levels. Results The proliferation of SKOV-3 cells was significantly suppressed by TSA combined with ATRA and the difference was significant compared with other groups （P〈0.05）. The apoptosis rate of combination group was higher than that of other groups and the difference was significant （P〈0.05）. Conclusion TSA combination with ATRA induces cytotoxicity in ovarian cancer cells in vitro,and the effect was much more significant than other groups.