小儿佝偻病诊断与骨碱性磷酸酶检测相关性分析

Analysis of the correlation between the diagnosis of rickets and the detection of bone alkaline phosphatase in children

目的探究小儿佝偻病诊断与骨碱性磷酸酶检测的相关性及检测价值。方法选取2014年10月至2016年1月湖南省儿童医院骨科收治住院的小儿佝偻病患儿62例作为观察组,选取同期本院健康体检儿童62例作为正常对照组,采用碘硝基四氮唑紫法检测骨碱性磷酸酶活性,采用偶氮砷法检测血钙情况,对骨碱性磷酸酶与影响因素进行相关分析。结果观察组骨碱性磷酸酶异常检出率为96.77%(60/62),显著高于正常对照组45.16%(28/62),差异有统计学意义(P<0.05);观察组血钙异常检出率为22.58%(14/62),显著高于正常对照组1.61%(1/62),差异有统计学意义(P<0.05)。Spearman相关性分析表明,惊厥、挑食、多汗以及缺少维生素D与碱性磷脂酸酶活性存在正相关(P<0.05)。结论小儿佝偻病诊断与骨碱性磷酸酶活性密切相关,由骨碱性磷酸酶检测能够完成对小儿佝偻病的诊断。

Objective To investigate the correlation between the diagnosis of rickets and the detection of bone alkaline phosphatase and the detection value. Methods A total of 62 cases of children with infantile rickets were selected in Hunan Childrens Hospital who were hospitalized from October 2014 to January 2016 as the observation group,and another 62 cases of children with normal physical examination were selected at the same time in our hospital as the normal control group. The activity of bone alkaline phosphatase was de-tected by iodonitrotetrazole violet method;blood calcium was detected by azo arsenic method;the correlation between bone alkaline phosphatase and influential factors was analyzed. Results The detection rate of bone alkaline phosphatase abnormality in observation group was 96. 77% （60/62） , which was significantly higher than that in normal control group（45. 16% ,28/62） ,the difference being statistically significant（P〈CO. 05）; the detection rate of serum calcium abnormality in observation group was 22. 58% （14/62） , which was signif-icantly higher than that in normal control groupd. 61 % , 1/62） , and the difference was statistically signifi- cant（P〈C0. 05） ; Spearman correlation analys is showed that there was a pos itive correlation between seizure, picky eaters,sweating and lack of vitamin D and alkaline phosphatase activity（P〈C0. 05）. Conclusion T h e diagnosis of rickets in children is closely related to the activity of bone alkaline phosphatase, and the diagno-sis of rickets can be completed by detection of bone alkaline phosphatase.