3种酶联免疫吸附试验检测乙型肝炎病毒前S1及S2抗原的比较

A comparative study on three methods of enzyme linked immunosorbent assay for detecting hepatitis B virus Pre S1 and S2 antigen

目的比较酶联免疫吸附试验(ELISA)的3种不同检测方式,选择最佳检测方式,用于临床诊疗。方法用Addcare ELISA800全自动酶免仪、TECAN freedom evolyzer全自动酶免分析系统、手工ELISA法等3种方式对质控品标本和患者血清标本进行乙型肝炎病毒前S1抗原(Pre-S1Ag)和前S2抗原(Pre-S2Ag)检测,并采用统计学方法对其结果进行分析。结果 3种方式检测Pre-S1Ag的批内精密度分别为4.73%、5.38%、11.87%,检测Pre-S2Ag的批内精密度分别为4.91%、5.04%、11.75%;检测Pre-S1Ag的批间精密度分别为6.63%、7.90%、13.26%,检测Pre-S2Ag的批间精密度分别为6.74%、7.81%、12.59%。灵敏度均为100.00%。结论 3种方法有非常好的一致性,均能较好地检测Pre-S1Ag和Pre-S2Ag,Addcare ELISA800全自动酶免仪精密度最好,可进一步提升临床检验质量。

Objective To compare the three different methods of enzyme linked immunosorbent assay（ELISA）,to select the best method for clinical diagnosis and treatment.Methods Addcare ELISA800,TECAN freedom evolyzer and manual ELISA method were used to detect hepatitis B virus Pre S1antigen（preS1Ag）hepatitis B virus Pre S2antigen（preS2Ag）in confrontation control product samples and serum specimens from patients with HBV,and the results were analyzed by statistical methods.Results The batch precisions of the three methods to detect pre-S1 Ag were 4.73%,5.38%,11.87%,the batch precisions of the three methods to detect pre-S2 Ag were 4.91%,5.04%,11.75%.The inter batch precisions of the three methods to detect pre-S1 Ag were 6.63%,7.90%,13.26%,the inter batch precisions of the three methods to detect pre-S2 Ag were 6.74%,7.81%,12.59%.All the sensitivities were 100.00%.Conclusion All the three methods have good consistency,which could be used in the detection of Pre-S1 Ag and Pre-S2 Ag.The precision of Addcare ELISA800 is the best,which could further improve the quality of clinical testing.