两面针的愈伤组织诱导与分化

Induction and Differentiation of Calli from Zanthoxylum nitidum

两面针具有很高的药用价值,但资源贫乏。利用组织培养技术获得种苗,为恢复两面针的资源打下基础。研究结果表明,以无根苗的茎段为外植体,MS+4.0 mg/L KT+2.0 mg/L NAA培养基较适宜愈伤组织诱导,诱导率达到74.84%。适宜的分化培养基是MS+6.0 mg/L KT+0.2 mg/L IBA。经过2次分化诱导,芽分化率达到36.46%,每块愈伤组织平均分化出3.30个芽。分化后的芽可以通过增殖培养提高芽的数量和质量。适宜的增殖培养基是MS+4.0 mg/L KT+0.8 mg/L NAA,培养8周的芽增殖系数达到5.00。适宜生根诱导的培养基是1/2MS+0.2 mg/L KT+1.0 mg/L IBA,生根率达到66.75%。本研究成功地从两面针愈伤组织中分化出芽和根,获得了高繁殖系数的组织培养技术。

Zanthoxylum nitidum is a woody liana plant with high value of medicine,but its resources is shortage. To recover the resources of Z. nitidum,germchits should be obtained by tissue culture. The results showed that the optimal medium for callus induction from explant of sterile stem segment was MS + 4. 0 mg/L KT + 2. 0 mg/L NAA with induction rate of 74. 84%. The optimal medium for bud differentiation was MS + 6. 0 mg/L KT + 0. 2 mg/L IBA. The bud differentiation rate was 36. 46% after two times of differentiation culture,and 3. 30 buds were regenerated from a piece of callus. The quantity and quality of regenerated bud could be improved and increased by proliferation culture. The optimal medium for bud proliferation was MS + 4. 0 mg/L KT + 0. 8 mg/L NAA,the rate of bud proliferation reached 5. 00 after8 weeks culture. The optimal medium for root differentiation was 1/2MS + 0. 2 mg/L KT + 1. 0 mg/L IBA with highest differentiation rate of 66. 75%. A tissue culture system with high proliferation rate was established by successfully differentiating buds and roots from calli of Z. nitidum.