摘要
本实验以去卵巢后慢性应激模型,探究锁阳乙酸乙酯提取物(ECS)改善慢性应激小鼠认知功能障碍的神经保护机制。采用Morris水迷宫检测小鼠空间学习记忆能力,Western Blot检测海马组织突触蛋白中突触囊泡蛋白(synaptophysin,Syn)和突触后致密物(Postsynaptic density protein 95,PSD-95)表达量,通过海马组织形态学改变进一步证明ECS的神经保护作用。Morris水迷宫结果显示雌鼠锁阳乙酸乙酯组穿越平台次数增加(P<0.01)。Western Blot结果显示,ECS可增加Syn(P<0.05)与PSD-95(P<0.05)蛋白表达量。HE染色结果显示ECS可减轻海马神经元损伤,改善海马CA1区锥体细胞形态。结果表明ECS能够增加去势后慢性应激小鼠突触蛋白中Syn与PSD-95的表达量,保护海马神经元,改善慢性应激所致学习记忆障碍。
The mice given chronic unpredictable stress after ovariectomized was used to observe the effect of Cynomoriumsongaricumethyl acetate extract( ECS) on cognitive dysfunction induced by chronic stress. The Morris water maze was applied to test the spatial learning and memory ability. Western blot assay was used to test the expression of Syn and PSD95 in the hippocampus. HE staining was used to observe morphological change of hippocampal neurons. The results showed that the explore time of targeted quadrant of mice from ECS group increased( P < 0. 01) in the Morris water maze test. In addition,ECS can improve the expression of Syn( P < 0. 05) and PSD-95( P < 0. 05) in Western blot assay.Compared with model group,the neurological form of pyramidal cells in hippocampus were improved by HE staining.Hippocampus nerve cell injury was improved. In conclusion,ECS can improve the expression of Syn and PSD-95,protect hippocampus nerve cell and improve the cognitive dysfunction.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第8期1302-1306,共5页
Natural Product Research and Development
基金
教育部新世纪优秀人才项目(NCET-13-0695)
国家自然科学青年基金(81102623)
