柠条锦鸡儿不同极性部位抗氧化及抗须癣毛癣菌活性初筛

Preliminary Screening for Antioxidant and Anti-Trichophyton mentagrophyte Activities of Different Polarity Extracts from Caragana korshinskii Kom.

本文对柠条锦鸡儿的花、叶、枝皮和根皮的乙醇提取物进行了极性部位的分离,并对各部位的提取物进行了抗氧化及抗须癣毛癣菌的活性筛选。抗氧化活性通过DPPH、ABTS、羟自由基(·OH)和超氧阴离子(O_2^-·)自由基的清除能力来评价,抗须癣毛癣菌活性通过抑菌圈实验来进行初步筛选。结果表明,以Vc为对照,柠条锦鸡儿花和叶的提取物具有很好的DPPH和ABTS自由基的清除能力,尤其是它们的乙酸乙酯部位;但所有的提取物对·OH和O_2^-·自由基的清除能力均较弱(P<0.001)。抑菌圈实验发现,花、叶和枝皮的乙酸乙酯部位具有一定的抗菌活性,其中枝皮的抗菌活性最好;通过测定生物量,当秋季采集枝皮乙酸乙酯部位提取物浓度为128μg/m L其抑菌率可达到50%。活性筛选结果说明柠条锦鸡儿的花、叶和枝皮具有一定的深入开发潜力。

In this study,the flowers,leaves,bark and root of Caragana korshinskii Kom. were separately extracted by ethanol. And then all the extracts were further fractionated by petroleum ether( Pe) and ethyl acetate( Et) in sequence.Those extracts were applied to screen the anti-oxidant and anti-fungal activities. The anti-oxidant activity was carried out by scavenging radicals of DPPH,ABTS,hydroxyl radical( ·OH) and superoxide anion( O_2^-·). Inhibition zone was employed to evaluate the anti-fungal activity of those extracts. The results suggested that compared with vitamin C( Vc) the flowers and leaves of C. korshinskii showed good anti-oxidant activity of scavenging radical of DPPH and ABTS,especially for their Et fractions. However,all parts of the extracts showed weaker activity of scavenging ·OH and O_2^-· than Vc( p < 0. 001). Screening for anti-fungal activity,the results showed that the Et fraction of flowers,leaves and bark are anti-fungal of Trichophyton mentagrophyte to some extent,especially the bark. Moreover,the inhibition ratio can be up to 50% when the concentration of the Et fraction of bark collected in autumn. Those consequences turned out that flowers and leaves of C. korshinskii can be a potential and exploitative resource.