黄地安消胶囊对改善2型糖尿病GK大鼠胰岛素抵抗的影响

Improving effects of Huangdi Anxiao Capsules on insulin resistance in type 2 diabetic GK rats

目的观察黄地安消胶囊(葛根、枇杷叶、三七,等)对2型糖尿病(T2DM)模型GK大鼠的血糖和胰岛素抵抗的影响及可能的效应机制。方法选取符合T2DM模型的GK大鼠纳入实验,随机分为模型组,罗格列酮(1.44 mg/kg)组,参芪降糖颗粒(1.08 g/kg)组,黄地安消胶囊高、中、低(12、6、3 g/kg)组以及Wistar大鼠正常组。各组连续给药6周后,测定各组大鼠的空腹血糖(FBG)、血清胰岛素(FINS)含有量、空腹血清血糖(FPG),并计算胰岛素抵抗指数(HOMA-IR)。对血清生化指标(TG、TC、HDL-C、LDL-C)、糖化血红蛋白(Hb A1c)、胰高血糖素样肽-1(GLP-1)及胰岛素样生长因子(IGF-1)进行测定。取胰腺组织,常规石蜡包埋并进行HE染色,观察其病理形态的改变。结果与正常组比较,模型组FBG、FPG、FINS、HOMA-IR、TG、TC、HDL-C、LDL-C、Hb A1c均显著升高(P<0.01),GLP-1、IGF-1表达量显著降低(P<0.01)。与模型组比较,黄地安消高、中、低剂量组FBG、FPG、FINS、HOMA-IR、TG、TC、HDL-C、LDL-C、Hb A1c均明显降低(P<0.05,P<0.01);GLP-1、IGF-1表达量显著增加(P<0.05,P<0.01),黄地安消高、中、低剂量组胰腺组织结构改变明显减轻。结论黄地安消胶囊能够降低GK大鼠空腹血糖,改善胰岛素抵抗,其机制可能与促进胰岛细胞的产生与增殖,改善胰岛细胞的功能,增加胰岛素的分泌有关。

AIM To observe the effects of Huangdi Anxiao Capsules（ Puerariae lobatae Radix,Eriobotryae Folium,Notoginseng Radix et Rhizoma,etc.） on blood glucose and insulin resistance in GK rats with type 2 diabetes mellitus（ T2DM） and its possible mechanism of action. METHODS GK rats with T2 DM included in the experiment were randomly divided into model group,rosiglitazone（ 1. 44 mg/kg） group,Shenqi Jiangtang Granules（ 1. 08 g/kg） group,Huangdi Anxiao Capsules high,medium and low（ 12,6,3 g/kg） group and normal control group of Wistar rats. After six weeks of consecutive administration,fasting blood glucose（ FBG）,serum insulin（ FINS）,fasting plasma glucose（ FPG）,and insulin resistance index（ HOMA-IR） were measured in all groups.Serum biochemical indexes of（ TG,TC,HDL-C,LDL-C）,glycosylated hemoglobin（ Hb A1c）,glucagon-like peptide（ GLP-1） and insulin-like growth factor（ IGF-1） were measured. The pancreatic tissue was obtained by routine paraffin embedding and HE staining to observe the pathological changes. RESULTS Compared with the normal group,FBG,FPG,FINS,HOMA-IR,TG,TC,HDL-C,LDL-C and Hb A1 c of the model group were significantly higher（ P〈0. 01）,and GLP-1 and IGF-1 expressions were markedly decreased（ P〈0. 01）. Compared with the model group,the levels of FBG, FPG, FINS, HOMA-IR, TG, TC, HDL-C, LDL-C and Hb A1 c in Huangdi Anxiao Capsules high-,medium-and low-dose group were significantly decreased（ P〈0. 05,P〈0. 01）; GLP-1 and IGF-1 expressions were markedly increased（ P〈0. 05,P〈0. 01）,compared with the model group,the structure changes of pancreatic tissue in Huangdi Anxiao Capsules high-,medium-and low-dose groups largely reduced. CONCLUSION Huangdi Anxiao Capsules can reduce GK rats fasting blood glucose,insulin resistance,and its mechanism may be related to promoting the emergence and proliferation of pancreatic islet cells,improving the function of islet beta cells,and increasing insulin secretion.