加味补阳还五汤对糖尿病大鼠血糖的影响及其抗氧化活性

Effect of Supplemented Buyang Huanwu Decoction on blood glucose in diabetic rats and its antioxidant activity

目的考察加味补阳还五汤(黄芪、当归尾、赤芍,等)对糖尿病大鼠血糖的影响及其抗氧化活性。方法建立链脲佐菌素(STZ)诱导糖尿病大鼠模型,以二甲双胍为阳性对照组。给药组灌胃加味补阳还五汤后,测定各组大鼠空腹血糖、血清及组织(心脏、肾脏、胰腺)中超氧化物岐化酶(SOD)活性、丙二醛(MDA)含有量。HPLC法测定血浆中抗氧化成分毛蕊异黄酮苷、芍药苷含有量,计算其药动学参数。结果与模型组比较,加味补阳还五汤组降血糖作用明显(P<0.05);血清及各组织(除胰腺外)中SOD活性显著提高,MDA含有量显著降低(P<0.05)。毛蕊异黄酮苷、芍药苷的药动学行为符合二室开放模型,血药质量浓度在50~70 min内达到最高,180~720 min内变化不明显。结论加味补阳还五汤可降低糖尿病大鼠血糖,并提高心脏和肾脏抗氧化活性。方中毛蕊异黄酮苷和芍药苷吸收较快,代谢较慢,有利于相关治疗。

AIM To investigate the effect of Supplemented Buyang Huanwu Decoction（ Astragali Radix,Angelica tail,Paeoniae Radix rubra,etc.） on blood glucose in diabetic rats and its antioxidant activity. METHODS The diabetic rat model induced by streptozotocin（ STZ） was established,with metformin as positive control group. After intragastric administration with Supplemented Buyang Huanwu Decoction,the fasting blood glucose,superoxide dismutase（ SOD） activity and malondialdehyde（ MDA） content in serum and tissues（ heart,kidney and pancreas） in rats were detected. HPLC was used to determine the contents of antioxidant constituents（ calycosin-7-O-β-D-glucoside and peoniflorin） in plasma, whose pharmacokinetic parameters were then calculated.RESULTS Compared with the model group,the hypoglycemic activity in the Supplemented Buyang Huanwu Decoction group was obvious（ P〈0. 05）,the SOD activity in serum and various issues（ except for pancreas） was significantly enhanced,together with significantly reduced MDA level（ P〈0. 05）. The pharmacokinetic behavior of two constituents（ calycosin-7-O-β-D-glucoside and peoniflorin） accorded with two-compartment open model,whose blood concentrations reached the highest within 50-70 min,and showed no obvious changes within 180-720 min.CONCLUSION Supplemented Buyang Huanwu Decoction can reduce the blood glucose in diabetic rats and improve the antioxidant activities in heart and kidney. The fast absorption and slow metabolism of calycosin-7-O-β-Dglucoside and peoniflorin in decoction are beneficial to related treatment.