一测多评法同时测定消癥微丸中4种柴胡皂苷

Simultaneous determination of four saikosaponins in Xiaozheng Pellets by QAMS

目的建立一测多评法同时测定消癥微丸(柴胡、香附、大黄等)中4种柴胡皂苷的含有量。方法该药物5%氨水-甲醇提取液的分析采用Waters Xbridge C18色谱柱(250 mm×4.6 mm,5μm);以乙腈-水为流动相,梯度洗脱;体积流量1.0 m L/min;检测波长210、254 nm。以柴胡皂苷a为内标,计算柴胡皂苷b1、b2、c的相对校正因子,再测定其含有量。结果 4种柴胡皂苷在各自范围内线性关系良好(r≥0.999 5),平均加样回收率98.08%~102.94%,RSD 0.85%~1.82%,一测多评法所得结果与外标法接近。结论该方法简单、精确、可行,可用于消癥微丸的质量控制。

AIM To establish a quantitative analysis of multi-components by single-marker（ QAMS） method for the simultaneous content determination of four saikosaponins in Xiaozheng Pellets（ Bupleuri Radix,Cyperi Rhizoma,Rhei Radix et Rhizoma,etc.）. METHODS The analysis of 5% ammonia-methanol extract of this drug was performed on a Waters Xbridge C18column（ 250 mm × 4. 6 mm,5 μm）,with the mobile phase comprising of acetonitrile-water flowing at 1. 0 m L/min in a gradient elution manner,and the detection wavelengths were set at210 nm and 254 nm. With saikosaponin a as a internal standard,the relative correction factors of saikosaponins b1,b2 and c were calculated,followed by the determination of their contents. RESULTS Four saikosaponins showed good linear relationships within their own ranges（ r ≥ 0. 999 5）,whose average recoveries were 98. 08%-102. 94% with the RSDs of 0. 85%-1. 82%. The results obtained by QAMS approximated those obtained by external standard method. CONCLUSION This simple,precise and feasible method can be used for the quality control of Xiaozheng Pellets.