TRAIL及其受体在膀胱癌中的表达及相关性研究

Expression and correlation of TRAIL and its receptors in bladder cancer

目的:观察肿瘤坏死因子相关诱导凋亡配体(TRAIL)及其受体DR4、DcR1在膀胱癌组织及正常膀胱黏膜中的表达,并探讨其与临床病理特征间的相关性。方法:采用免疫组织化学PV9001二步法检测64例膀胱癌石蜡标本及14例正常膀胱组织中的TRAIL、DR4、DcR1的表达,结合患者病理资料,分析其与患者临床病理特征之间的关系。结果:TRAIL、DR4在膀胱癌组织中的表达较正常组织中的表达低,DcR1表达与之相反(P<0.05)。进一步统计分析得出TRAIL、DR4在高分化膀胱癌中表达较中低分化癌高,DcR1与之相反(P<0.05)。TRAIL、DR4、DcR1的表达与性别、年龄、临床分期及是否淋巴转移等病理特征无关(P>0.05)。结论:TRAIL、DR4、DcR1在膀胱癌组织及正常组织中的表达具有统计学差异,并且与病理分级具有一定的相关性,与其他临床病理特征无关。

Objective To observe the tumor necrosis factor related apoptosis inducing ligand （TRAIL） and its re-ceptor DR4 and DcRl expression in bladder carcinoma and normal bladder mucosa,and to investigate its correlation with the clinicopathological features. Methods ： Immunohistochemistry PV9001 two step method was used to detect 64 cases of bladder cancer specimens and 14 cases of normal bladder tissue in TRAIL, DR4, DcR1 , combined with the pathological data of patients,analyze their relationship with the clinicopathological characteristics of patients. Results：The expression of DR4 and TRAIL in bladder cancer tissues was lower than that in normal tissues,but DcRl expres-sion was opposite （P 〈0. 05） . Further statistical analysis showed that DR4 and TRAIL expression in highly differenti-ated bladder cancer was lower than that of moderately and pcorly differentiated carcinoma, and DcRl was the opposite （P 〈0. 05） . Expression of TRAIL,DR4 and DcRl was not related to gender,age,clinical stage,lymph node metasta-sis and other pathological features（P 〉0. 05）. Conclusion： The expression of TRAIL, DR4, DcRl in bladder cancer tissue and normal tissue is statistically significant, and has a certain correlation with pathological grading, which is not related to other clinical pathological characteristics.