重复经颅磁刺激对阿尔茨海默病大鼠脑电LZ复杂度和脑白质中碱性髓鞘蛋白分布的影响

Effects of rTMS on the LZ complexity of EEG signals and distribution of MBP in AD rats

目的探讨重复经颅磁刺激(r TMS)对阿尔茨海默病(AD)大鼠学习记忆能力影响的可能机制。方法挑选学习记忆无差异的SD大鼠90只,随机挑选30只作为对照组;大鼠双侧海马区注射Aβ1-42建立AD模型,用Morris水迷宫和免疫组化验证;将造模成功的大鼠随机分为AD组和(r TMS+AD)组,各组30只;(r TMS+AD)组施加r TMS,对照组和AD组施加伪刺激;干预完成后,进行水迷宫实验、脑电采集与分析、免疫组化实验以及统计分析。结果 (1)AD组较对照组大鼠逃避潜伏期变长,距离平台平均距离变远;(r TMS+AD)组较AD组大鼠逃避潜伏期变短,距离平台平均距离减小,差异有统计学意义(P<0.05)。(2)AD组较对照组大鼠脑电LZ复杂度降低,(r TMS+AD)组较AD组大鼠脑电的LZ复杂度升高,差异有统计学意义(P<0.05)。(3)对照组脑组织未见斑块状沉淀,细胞形态完好,轮廓清晰,排列整齐,髓鞘着色较深,分布长且连续;AD组脑组织中出现斑块状沉淀,细胞萎缩聚集,轮廓模糊,排列混乱,髓鞘染色较浅,分布短且不连续;(r TMS+AD)组较AD组脑组织中细胞萎缩情况减轻,髓鞘染色变深,分布增加。结论 r TMS可增加脑组织中髓鞘分布,提高AD大鼠脑电LZ复杂度,改善AD大鼠的学习记忆能力。

Objective To explore the possible mechanism of repetitive transcranial magnetic stimulation(r TMS)in learning and memory ability of Alzheimer′s disease(AD)rats.Methods 90 SD rats with no difference in learning and memory were selected and 30 rats were randomly selected as control group.The rest 60 were used for establishment of AD model by injecting A beta 1-42 into bilateral hippocampus of rats,which was verified by Morris water maze test and immunohistochemistry.The successfully prepared model rats were randomly divided into AD group and(r TMS+AD)group,30 rats in each group.The(r TMS+AD)group was treated with rTMS,and the control group and AD group with pseudo stimulation.After intervention,Water maze test,EEG acquisition and analysis,immunohistochemistry and statistical analysis were done.Results (1)Compared with control group,the escape latency of AD group was longer and the average distance from the platform was farther away.Compared with AD group,the escape latency of(r TMS+AD)group was significantly longer and the average distance from the platform was significantly farther away(P<0.05).(2)Compared with control group,the LZ complexity of EEG signals in AD group was decreased.Compared with the AD group,the LZ complexity of EEG signals in(r TMS+AD)group was significantly increased statistically(P<0.05).(3)The results of immunohistochemistry showed that there was no plaque in the brain tissues of control rats.The morphology of the cells was clear,and the cells arranged neatly.Myelin staining was deeper and the distribution was long andconnected.There was plaque in the brain tissues of AD rats.Brain tissue cells became atrophy,the morphology of the cells was not clear,and the cells arranged randomly.Myelin staining was lighter and the distribution was short and disconnected.Compared with AD group,the cell atrophy of(r TMS+AD)group was reduced,and Myelin staining became deeper.Conclusions r TMS can increase the distribution of myelin in brain tissue,raise the LZ complexity of EEG signals in AD model rats,and improve the learning and memory ability of them.