血清DCD、CA199、CA724和CEA联合检测对大肠癌的诊断价值

Value of combined detection of serum DCD, CA199, CA724, and CEA in diagnosis of colorectal cancer

目的探究联合检测皮离蛋白(DCD)、糖类抗原199(CA199)、糖类抗原724(CA724)与癌胚抗原(CEA)肿瘤标志物对大肠癌早期诊断的可能性。方法 81例明确诊断的大肠癌患者作为病例组,同期纳入30例健康体检者作为对照组,采用ELISA方法检测血清中DCD的表达情况,并采用电化学发光免疫分析法检测血清中CEA、CA199、CA724的水平,采用Western blot检测DCD在大肠癌组织及癌旁组织中的表达。结果病例组血清中CA199、CA724以及CEA的浓度明显高于对照组,差异有统计学意义(P<0.001);诊断大肠癌的ROC曲线下面积:CA199、CA724、CEA联合诊断为0.930,DCD、CA199、CA724、CEA联合诊断是0.942,均高于单独使用时的曲线下面积;病例组TNM分期越高,血清中CA199、CEA的表达量越高,且差异有统计学意义(P<0.001),DCD在大肠癌组织中的表达浓度明显高于癌旁组织,差异有统计学意义(P<0.05)。结论大肠癌组织DCD表达水平明显高于癌旁组织,血清中DCD、CA199、CA724以及CEA的表达有可能作为肿瘤联合诊断的标志物,对大肠癌的辅助诊断有较高的临床应用价值。

Objective To investigate the possibility of early diagnosis of colorectal cancer by combined detection of tumor markers dermcidin(DCD),CA199,CA724 and CEA.Methods We selected 81 colorectal cancer patients and 30 healthy individuals.Serum levels of DCD were detected by ELISA,and serum levels of CA199,CA724 and CEA were detected by electrochemical luminescent immunoassay,and the expression of DCD in colorectal cancer tissue and para-carcinoma tissue was measured by Western blot.Results Serum levels of CA199,CA724,and CEA were significantly higher in colorectal cancer patients than in healthy individuals(P<0.001).Combined detection of DCD,CA199,CA724 and CEA had a greater AUC(0.942)for identifying colorectal cancer from the controls.The expression of DCD was significantly higher in colorectal tissue than in para-carcinoma tissue(P<0.05).Conclusions Level of DCD expression was significantly higher in colorectal tissue than in para-carcinoma tissue.Expressions of serum DCD,CA199,CA724,and CEA may be a marker in diagnosis of cancer,with higher clinical value for the adjuvant diagnosis of colorectal cancer.