严重脓毒症发病中血清超氧化物歧化酶与CD4^+CD25^+Treg细胞的相关性

The correlation of serum SOD and CD4^+CD25^+ regulatory T cell in the mechanism of severe sepsis

目的观察严重脓毒症患者早期氧化应激和免疫抑制状态,并探讨其相关性。方法前瞻性随机对照研究,设立病例组(n=51)和对照组(n=31),分别采用ELISA法测定血清超氧化物歧化酶(SOD)浓度,流式细胞技术测定外周血CD4+CD25+Treg%,比较两组之间的差异,探讨病例组各参数间的相关性。结果病例组血清SOD浓度[36.0(15.0,50.9)ng/mL]显著低于对照组[60.3(40.2,88.2)ng/mL](P<0.01),而CD4+CD25+Treg%则显著高于对照组[6.49(4.5,11.63)vs.5.36(3.92,6.39),P<0.01],提示病例组存在显著的氧化应激和免疫抑制。病例组血清SOD浓度和CD4+CD25+Treg%未发现显著相关性。结论严重脓毒症患者早期存在免疫抑制和氧化应激。

Objective To evaluate the condition of oxidative stress and immunosuppression in early stage of severe sepsis,and investigate the correlation between them.Methods A prospective random control study included patients group(n=51)and control group(n=31).The concentration of serum superoxide dismutase was measured by enzyme linked immunosorbent assay(ELISA),CD4^+CD25^+Treg% was measured by flow cytometry,respectively.The difference between two groups was compared and the correlation between parameters in patients group was evaluated.Results The concentration of serum SOD was lower than control group(P<0.01).CD4^+CD25^+Treg% significantly high,compared to the control group(P<0.01).There was no strong correlation between parameters in patients group.ConclusionOxidative stress and immunosuppression are exist in the early stage of severe sepsis.