CRISPR/Cas9技术用于清除乙型肝炎病毒的研究进展

Application of CRISPR/Cas9 technology in hepatitis B virus clearance

乙型肝炎病毒(HBV)慢性感染作为中国乃至全球的重大健康问题,使乙肝患者长期处于肝硬化及肝癌的巨大威胁中。乙型肝炎病毒共价闭合环状DNA(ccc DNA)是HBV在肝细胞内复制中必不可少的一环,是乙肝彻底治愈的关键。然而,目前针对乙肝的治疗药物尚无法彻底清除肝细胞内的ccc DNA,且服药周期长、副作用大。利用新兴的基因编辑技术CRISPR/Cas9,人工设计靶向HBV系统可高效清除潜藏在胞内的ccc DNA,为乙肝的彻底治愈带来希望。文章就CRISPR/Cas9技术用于清除HBV的研究进行综述。

Chronic hepatitis B virus（HBV） infections remain a major public health problem worldwide,which puts human at high risks of liver cancer and liver cirrhosis. The stable nuclear covalently closed circular DNA（ccc DNA） is an important step of the HBV replication cycle,as well as the key of drastically destroy the HBV. However,α-interferons and oral nucleo（s） tide analogs cannot target and destroy the ccc DNA. Nowadays,clustered regularly interspaced short palindromic repeats（CRISPR）/Cas9 gene editing technology can specifically target the HBV ccc DNA,which brings hope to conquer HBV. Researches of CRISPR/Cas9 technology applied to clear HBV have been reviewed in this paper.