车前草中总苯乙醇苷及毛蕊花糖苷的含量测定

Determination of Total Phenylethanoid Glycosides and Acteoside in Plantago Herba

目的:测定车前草中总苯乙醇苷及毛蕊花糖苷的含量,为评价药材质量提供科学依据。方法:以毛蕊花糖苷为对照,用紫外-可见分光光度(UV)法测定车前草中总苯乙醇苷的含量,用高效液相色谱(HPLC)法测定毛蕊花糖苷的含量,色谱条件:以色谱柱:ODS2 C_(18)(150 mm×4.6 mm,5μm);流动相:乙腈-0.1%甲酸溶液(13∶87);流速:1 ml·min^(-1),检测波长:332nm,柱温:30℃,进样量:10μl。结果:UV法:对照品溶液和样品溶液在332 nm处有最大吸收,在0.003 1~0.155 0μg·ml^(-1)范围内浓度与吸光度呈良好的线性关系(r=0.999 5),3批样品中总苯乙醇苷的含量分别为2.73%、2.61%、2.84%。HPLC法:毛蕊花糖苷在0.000 6~0.155 0μg·ml^(-1)范围内与峰面积呈良好的线性关系(r=0.999 1),加样回收率为98.5%,RSD为1.6%(n=6),3批样品中毛蕊花糖苷含量分别为0.54%、0.51%、0.56%。结论:该方法操作简单、稳定性好、重复性较好,可用于车前草中总苯乙醇苷及毛蕊花糖苷的含量测定。

Objective： To determine total phenylethanoid glycoside and acteoside in Plantago Herba to provide reference for evalu- ating the quality of medicinal materials. Methods： With acteoside as the control sample, a UV visible spectrophotometric method was used to determine total phenylethanoid glycosides in Plantago Herba. An HPLC method was applied to determine acteoside in Plantago Herba, and the conditions were as follows： an ODS2 C18 （ 150 mm×4.6 mm,5μm） chromatographic column was used with acetonitrile 0.1% formic acid （13 ： 87） as the mobile phase at a flow rate of 1.0 ml min-1 , the detection wavelength was 332nm, the column temperature was 30℃, and the sample volume was 10 μl. Results： The reference solution and the sample solution had the maximum absorption at 332 nm, and the linear relationship was good within the range of 0.003 1.0. 155 0 mg ml- 1（r = 0.999 5 ）. The content of total benzene alcohol glycosides in 3 batches of samples was 2.73%, 2.61% and 2.84%, respectively; acteoside over the range of 0.000 6-0. 155 0 mg ml-1（ r = 0.999 1 ） showed a good linear relationship with peak area, the sample recovery was 98.5% and the RSD was 1.6% （n =6）, and the acteoside content in 3 batches of samples respectively was 0.54%, 0.51% and 0.56%. Conclusion： The method is simple, accurate and reproducible, and can be used for the determination of total phenylethanoid glycosides and acteoside in Plantago Herba .