阿咖酚散中游离水杨酸检查方法的改进

Improved Determination Method for Free Salicylic Acid in Paracetamol,Caffeine and Aspirin Powder

目的:建立HPLC法检查阿咖酚散中的游离水杨酸。方法:色谱柱Welch Ultimate~? C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-四氢呋喃-冰醋酸-水(22∶5∶5∶68),检测波长为303 nm,流速为1.0 ml·min^(-1)。结果:线性范围为1.500~75.000μg·ml^(-1)(r=0.999 9),平均回收率为99.12%,RSD为0.75%(n=9),检出限为1.5 ng(S/N=3)。结论:本方法简便准确、灵敏度高,稳定性比现行标准大幅提高,可用于阿咖酚散中游离水杨酸的检查。

Objective： To establish a determination method for free salicylic acid in paracetamol, caffeine and aspirin powder by HPLC. Methods： The column was Welch Ultimate C18 （250 mm ×4.6 mm,5μm）, the mobile phase was acetonitrile-tetrahydrofu- ran-glacial acetic acid-water（22：5：5：68）, the detection wavelength was 303 nm, and the flow was 1. 0 ml min-1. Results： The linear range of hydrochloride was 1. 500-75. 000 μg ml - i （ r = 0. 999 9 ）, the average recovery was 99.12% with RSD of 0.75% （ n =9 ）, and the detection limit was 1.5 ng（ S/N=3 ）. Conclusion： The method is simple, the result is accurate with higher sensitivity and the better reproducibility, which can be used for the determination of free salicylic acid in paracetamol, caffeine and aspirin powder.