芒果叶提取物芒果苷安全性评价Ⅰ:安全药理学试验

Pharmacological Study of Safety of Mangiferin Extracted from Mango Leaf

目的:观察芒果苷对神经系统、心血管系统和呼吸系统的影响,明确其一般药理活性,为后续毒理试验提供参考。方法:对小鼠一次按0.24,1.0,3.0 g/kg体重灌胃给予芒果苷后,观察其一般行为表现、姿势、步态、有无流涎、流汗、暴燥、抽搐、肌颤等变化,测定其自主活动次数、协调运动情况和阈下睡眠剂量戊巴比妥钠对小鼠睡眠的影响;对Beagle犬一次按0.1,0.7,4.0 g/kg体重十二指肠给予芒果苷后,观测给药后180 min内心电图、血压、呼吸的改变。结果:小鼠一般行为表现、姿势、步态未见有明显改变,也未见有流涎、流汗、暴燥、抽搐、肌颤等现象;自主活动、协调运动情况和戊巴比妥钠阈下睡眠剂量小鼠入睡率均无明显影响。犬心电图、血压、呼吸均无明显变化。结论:在本实验条件下,芒果苷对小鼠精神和神经系统,对犬心血管系统和呼吸系统均无明显影响。

Objective： To observe the effect of mangiferin on nervous system,cardiovascular system and respiratory system,and analyze its general pharmacological effect. Thus providing reference for subsequent toxicological test. Methods： The mice were intragastric administered magiferin of 0. 24,1. 0 and 3. 0 g/kg · bw/Then changes in its general behavior,posture,gait,salivation,sweating,bruising,and convulsions were recorded. The effects of pentobarbital sodium on mice＇s sleep were measured. Also,beagle dogs were given mangiferin by duodenum in the dose of 0. 1,0. 7,4. 0 g/kg·bw. Changes in electrocardiogram,blood pressure,and respiratory were recorded after administration. Results： There was no significant change on the general behavior,posture,salivation,sweating,dryness,convulsions,muscle fibrillation of rats. There was no significant effect on independent activities,coordination of exercise and the sleep rate of mice on the sub-threshold sleep dose of pentobarbital sodium. Similarly,there was no significant change on dog electrocardiogram,blood pressure,and breathing. Conclusion： Under the experimental conditions,mangiferin has no significant effect on the mental and nervous system of mice or the cardiovascular system and respiratory system of dog.