寻常型银屑病中医三证型形成与皮肤组织中JAK1/STAT3信号通路关系的研究

Relationship Between Three TCM Syndromes of Psoriasis and JAK/STAT Signaling Pathway

目的:探讨寻常型银屑病血热、血瘀、血燥证形成与皮肤组织中JAK1/STAT3信号通路的关系。方法:选取2013年10月至2014年10月东直门医院皮肤科门诊和病房收治的寻常型银屑病患者18例(严格中医辨证分为血热证、血瘀证、血燥证三型),正常人受试者6例,记录患者一般情况,用RT-PCR法检测各组受试者皮肤组织中IL-22R1、JAK1、STAT3、Cmyc、VEGF的mRNA表达差异。结果:检测24例受试者皮肤组织,IL-22R1、JAK1、STAT3、C-myc的mRNA表达相互间均呈正相关,差异有统计学意义(P<0.05或P<0.01);血热证患者皮损中,IL-22R1、JAK1、STAT3、C-myc的mRNA表达均明显高于正常人,差异有统计学意义(P<0.01),血燥证IL-22R1、JAK1、STAT3的mRNA表达高于正常人,差异有统计学意义(P<0.05)。结论:JAK1/STAT3信号通路的异常活跃,可能是银屑病血热证临床表现形成的重要因素;且JAK/STAT信号通路活化差异可能与血热证向血瘀、血燥证转变的过程相关。

Objective： To observe the differences between blood heat syndrome,blood dryness syndrome and blood stasis syndrome of psoriasis in JAK/STAT signaling pathways. Methods： A total of 18 psoriasis cases in our hospital were divided into 3 groups based on TCM syndrome differentiation. The three groups were blood heat syndrome group,blood dryness syndrome group and blood stasis syndrome group. And 6 healthy participants also participated in the trail. Their general information was recorded. RTPCR was employed to test the difference of IL-22R1,JAK1,STAT3 and c-myc in participants＇ skin tissue samples. Results： The gene transcription correlation test among 24 psoriasis patients show that IL-22R1,JAK1,STAT3 and c-myc were all positively correlated to each other（ P〈0. 05 or P〈0. 01）. The gene transcriptions of IL-22R1,JAK1,STAT3 and c-myc in blood heat group were significantly higher than healthy case group（ P〈0. 01）. In blood dryness group,gene transcription of IL-22R1,JAK1 and STAT3 were higher than healthy group（ P〈0. 05）. Conclusion： The active status of JAK/STAT signaling pathway could be an important factor in the formation of psoriasis blood heat syndrome. While the JAK/STAT signaling pathway may result in blood heat syndrome turning into blood dryness syndrome and blood stasis syndrome.