纯钛超声工作尖与传统超声工作尖对钛表面形态及人牙周膜细胞黏附强度影响的比较研究

Evaluation of the surface alterations and the adhesion strength of human periodontal ligament cells on titanium surface treated by pure titanium and conventional ultrasonic scaler tips

目的比较经纯钛超声工作尖与传统不锈钢超声工作尖刮治后的钛表面形态变化及人牙周膜细胞(human periodontal ligament cells,hPDLCs)对不同钛片表面的黏附强度。方法将60个实验所用钛片随机分为5组,每组12个。Ti10组:用纯钛超声工作尖刮治钛片表面10s;Ti30组:用纯钛超声工作尖刮治钛片表面30s;Cv10组:用传统超声工作尖刮治钛片表面10s;Cv30组:用传统超声工作尖刮治钛片表面30s;C组:钛片表面不做任何处理,作为对照。刮治后使用扫描电镜(scanning electron microscope,SEM)观察各组钛片表面的形态变化,再用表面粗糙度轮廓仪测量钛片表面粗糙度;将hPDLCs与处理后的各组钛片共同培养12h后,利用CCK-8法检测处理后钛片有无细胞毒性及hPDLCs的黏附强度。结果 SEM和表面粗糙度轮廓仪显示,经过表面处理后,Ti10组钛片表面粗糙度明显小于Cv10组(P<0.05),但增加处理时间至30s后各组之间的粗糙度差别无统计学意义(P>0.05)。细胞毒性实验表明,处理后的钛片无细胞毒性。处理后钛片的细胞黏附实验表明,hPDLCs对两种材料工作尖处理后的钛表面的黏附强度差异无统计学意义(P>0.05)。结论纯钛超声工作尖相比传统超声工作尖,在刮治过程中对钛表面的保护具有一定优势,但对刮治后的细胞黏附的促进,未显示出明显的优势。

Objective To compare the effects of pure titanium and conventional steel ultrasonic scaler tips on titanium surface and evaluate the adhesion strength of human periodontal ligament cells （hPDLCs）on the titanium surface treated by the two different tips.Methods Totally 60 smooth titanium discs were used in this research.The discs were randomly divided into five groups,each of which contained 12 discs.Ti10 group：discs were scaled for 10s with pure titanium tip;Ti30 group：discs were scaled for 30s with pure titanium tip;Cv 10 group：discs were scaled for 10s with conventional steel tip;Cv30 group：discs were scaled for 30s with conventional steel tip.Control group：discs were not scaled.After the treatment according to the protocols,the morphology and roughness of the discs were evaluated by scanning electron microscope （SEM）and profilometer separately.In order to test the biocompatibility of the treated titanium discs,hPDLCs were co-cultured with those discs for 12 hours,and the cell toxicity of treated discs and the adhesion strength of hPDLCs on titanium discs was calculated with CCK-8 method.Results SEM results showed that Ti10 group had less morphological changes than Cv10 group and this change was highly consistent with the profilometer results which also showed that the roughness of titanium at Ti10 group was significantly lower than Cv10 group （P 〈 0.05）.However,if the scaling time increased to 30 seconds,there were no statistical difference among groups.There were no significant difference among groups for the adhesion strength of hPDLCs after being treated by different scaler tips and no toxicity was found.Conclusion Pure titanium ultrasonic scaler tips are better than conventional ultrasonic scaler tip with respect to the protection of titanium surface,although there were no significant differences in the promotion of cell adhesion.