强直性脊柱炎患者血清非磷酸化基质γ-羧基谷氨酸蛋白和巨噬细胞集落刺激因子水平与骨代谢生物化学指标的相关性

Correlation of the serum levels of circulating non-phosphorylated matrix Gla protein,macrophage colony stimulating factor and biochemical indexes of bone metabolism in patients with ankylosing spondylitis

目的研究强直性脊柱炎患者血清非磷酸化基质γ-羧基谷氨酸蛋白(dp MGP)、巨噬细胞集落刺激因子(M-CSF)水平与骨代谢生物化学指标的相关性。方法选择信阳市中心医院2014年4月至2016年8月收治的强直性脊柱炎患者82例为观察组,另选取体检健康者54例作为对照组。采用双抗体夹心酶联免疫吸附试验测定2组患者血清中M-CSF、骨碱性磷酸酶(BALP)、骨钙素(OC)水平,采用竞争性酶联免疫吸附试验检测血清dp MGP水平,分析dp MGP、M-CSF与BALP、OC的相关性。结果观察组患者血清中dp MGP、M-CSF水平高于对照组(P<0.05);2组研究对象血清中BALP、OC水平比较差异无统计学意义(P>0.05)。2组研究对象血清dp MGP水平与BALP、OC无相关性(r=-0.326、0.427,P=0.084、0.125);血清M-CSF水平与BALP、OC呈负相关(r=-0.357、-0.538,P=0.043、0.047)。结论强直性脊柱炎患者血清M-CSF水平与BALP、OC呈负相关,可通过检测M-CSF水平来判断骨代谢情况。

Objective To investigate the correlation of the levels of serum circulating non-phosphorylated matrix Gla protein（ dp MGP）,macrophage colony stimulating factor（ M-CSF） and biochemical indexes of bone metabolism in patients with ankylosing spondylitis. Methods Eighty-two patients with ankylosing spondylitis in the Central Hospital of Xinyang City from April 2014 to August 2016 were selected as observation group; fifty-four healthy person were selected as control group. The M-CSF,bone alkaline phosphatase（ BALP）,osteocalcin（ OC） levels in the serum of patients in the two groups were determined by double antibody sandwich enzyme method; the dp MGP level in the serum was measured by competitive enzyme linked immunosorbent assay method. The correlation between dp MGP,M-CSF and BALP,OC were analysed. Results The levels of serum dp MGP and M-CSF of patients in the observation group were significantly higher than those in the control group（ P〈0. 05）; there was no significant difference in the levels of serum BALP and OC between the two groups（ P〉0. 05）. The level of serum dp MGP of all subjects was not correlated with BALP and OC（ r =-0. 326,0. 427; P = 0. 084,0. 125）; the level of serum M-CSF was negatively correlated with BALP and OC（ r =-0. 357,-0. 538; P = 0. 043,0. 047）. Conclusion The patients with ankylosing spondylitis have abnormal bone metabolism. The serum level of M-CSF is negatively related to BALP and OC in ankylosing spondylitis,and the level of M-CSF can reflect the state of bone metabolism.