磷脂酸磷酸酶2域1A在不同子宫颈癌细胞中的表达

Expression of phosphatidic acid phosphatase type 2 domain containing 1A in different human cervical carcinoma cells

目的探讨磷脂酸磷酸酶2域1A(PPAPDC1A)在人4种子宫颈癌细胞中表达的差异。方法通过实时荧光定量聚合酶链式反应和Western blot法分别检测PPAPDC1A mRNA和蛋白在人子宫颈癌C33-A细胞、Caski细胞、HeLa细胞和SiHa细胞中的表达。结果子宫颈癌SiHa、HeLa、Caski和C33-A细胞中PPAPDC1A mRNA的表达量分别为0.344±0.027、0.593±0.017、0.899±0.030和2.920±0.045,PPAPDC1A蛋白的表达分别为0.514±0.030、1.185±0.096、1.379±0.084和1.761±0.072;子宫颈癌SiHa、HeLa、Caski和C33-A细胞中PPAPDC1A mRNA和蛋白的表达比较差异有统计学意义(P<0.05)。PPAPDC1A mRNA和蛋白在4种子宫颈癌细胞中表达的顺序为:C33-A细胞>Caski细胞>HeLa细胞>SiHa细胞。结论 PPAPDC1A基因在不同子宫颈癌细胞中存在差异表达。

Objective To investigate the expression difference of phosphatidic acid phosphatase type 2 domain containing 1 A （PPAPDC1 A） in four kinds of human cervical carcinoma cells.Methods Real-time quantitative polymerase chain reaction （qRT-PCR） and Western blot methods were used to detect the expressions of PPAPDC1A mRNA and protein in cervical carcinoma C33-A cells,Caski cells,HeLa cells and SiHa cells.Results The expression of PPAPDC1A mRNA in SiHa cells,HeLa cells,Caski cells and C33-A cells was 0.344 ± 0.027,0.593 ± 0.017,0.899 ± 0.030 and 2.920 ± 0.045,respectively;the expression of PPAPDC1A protein in SiHa cells,HeLa cells,Caski cells and C33-A cells was 0.514 ± 0.030,1.185 ± 0.096,1.379 ± 0.084 and 1.761 ± 0.072,respectively.There were significant differences in the expressions of PPAP-DC1 A mRNA and protein in the four kinds of cervical carcinoma cells （P 〈 0.05）.The order of PPAPDC1A mRNA and protein expressions in the four kinds of cervical carcinoma cells was as follows：C33-A cells 〉 Caski cells 〉 HeLa cells 〉 SiHa cells.Conclusion PPAPDC1A gene is differentially expressed among C33-A cells,Caski cells,HeLa cells and SiHa cells.