龙眼多糖通过下调氧自由基水平的抗疲劳作用研究

Effect of Polysaccharides of the Euphoria Longan(Lour.)Steud in Antifatigue by Reducing the Level of Oxygen Free Radicals

为了探讨龙眼多糖抗运动性疲劳的作用及其抗氧化调节机制,本文以小鼠作为实验对象,观察小鼠负重力竭游泳时龙眼多糖抗疲劳作用。通过紫外分光光度法,检测龙眼多糖对超氧阴离子(O-2·)和羟自由基(·OH)的清除作用;测定小鼠游泳后血液中丙二醛(MDA)含量、尿素氮(BUN)含量、乳酸脱氢酶(LDH)活性、超氧化物歧化酶(SOD)活性、乳酸变化等。结果表明:龙眼多糖能明显延长小鼠负重游泳时间(P<0.05),减少小鼠尿素氮的产生(P<0.05)以及乳酸含量(P<0.05)和丙二醛(MDA)含量(P<0.05),增加乳酸脱氢酶(LDH)(P<0.05)和超氧化物歧化酶(SOD)(P<0.05)的活性。龙眼多糖对羟自由基(·OH)有较强的清除作用,对超氧阴离子自由基(O-2·)的清除作用较小,可见,龙眼多糖具有良好的抗疲劳作用。

The purposes of the experiments are to explore the effect of polysaccharides of the Euphoria Longan（Lour.）Steud in resisting sport fatigue and its mechanism for anti-oxidation.The weighbearing swimming time span of the mouse was observed to explore the effect of polysaccharides of the Euphoria Longan（Lour.）Steud in resisting sport fatigue;ultraviolet spectrophotometry was adopted to explore the effect of polysaccharides of the Euphoria Longan（Lour.）Steud in eliminating O2-· and ·OH.The MDA,BUN,LDH,SOD and lactic acid of the mouse after the weigh-bearing swimming were observed to explore the mechanism of polysaccharides of the Euphoria Longan（Lour.）Steud for antifatigue.The findings are as follows,polysaccharides of the Euphoria Longan（Lour.）Steud can prolong the time span of weight-loaded swimming in the mouse（P〈0.05）,reduce the production of BUN（P〈0.05）and the accumulation of lactic acid（P〈0.05）and MDA（P〈0.05）,and activate LDH（P〈0.05）and SOD（P〈0.05）.Polysaccharides of the Euphoria Longan（Lour.）Steud can eliminate·OH greatly but its effect in eliminating O2-· is not so obvious.These show that polysaccharides of the Euphoria Longan（Lour.）Steud is effective in resisting fatigue.