激光共焦显微拉曼光谱技术在人舌鳞癌细胞检测中的应用

Micro-Raman spectroscopy technique in the detection of human tongue squamous carcinoma cell

目的:采用激光共焦显微拉曼光谱技术对人舌鳞癌细胞CAL-27进行检测,并将其与正常人体口腔黏膜上皮细胞(NHOK)进行对比和分析,从而从分子层面区分CAL-27和NHOK。方法:培养CAL-27和NHOK,并用激光共焦显微拉曼光谱对其进行检测,以及采用主成分分析法和线性判别技术对其进行分析。结果:对比CAL-27和NHOK光谱差谱,发现谱宽于735、959、1 334、1 575 cm-1处,CAL-27的峰值比NHOK强,但在1 033 cm-1处,则相对要弱。结合主成分分析和线性判别技术的灵敏度为96.8%,特异性为90.3%,诊断率为93.5%。受试者操作特性曲线下面积为0.971。结论:采用激光共焦显微拉曼技术可区分CAL-27和NHOK。

Objective To detect human tongue squamous carcinoma cell CAL-27 with micro-Raman spectroscopy technique,and distinguish CAL-27 from normal human oral keratinocytes（NHOK） in the molecular level by comparing CAL-27 with NHOK. Methods The cultivated CAL-27 and NHOK were detected with micro-Raman spectroscopy technique, and analyzed using principal component analysis combined with linear discriminate analysis（PCA-LDA）. Results Comparing with NHOK, higher peak values were found at 735, 959, 1 334, 1 575 cm^（-1）, and lower peak value at 1 033 cm^（-1） in CAL-27.The PCA-LDA achieved a sensitivity of 96.8%, a specificity of 90.3%, and a diagnostic accuracy of 93.5%. The area under the receiver operating characteristic curve was 0.971. Conclusion Micro-Raman spectroscopy with PCA-LDA has the potential for distinguishing CAL-27 from NHOK.