响应面法优化纤维素酶辅助提取淫羊藿多糖工艺

Optimization of Epimedium Polysaccharide by Response Surface Methodology

为优化淫羊藿多糖的提取工艺,采用纤维素酶辅助热水浸提法从淫羊藿中提取淫羊藿多糖,在单因素试验结果的基础上,选择酶浓度、酶提温度、酶提时间为自变量,采用三因素三水平的Box-Behnken响应面设计,优化淫羊藿多糖的提取工艺。试验结果表明,淫羊藿多糖酶辅助提取的最佳工艺参数为:酶浓度110 U/μL,酶提温度60℃,酶提时间100 min,且酶提温度对多糖提取率的影响最大。在最佳优化条件下淫羊藿多糖提取率为10.06%,接近模型的最大预测值(10.199%)。试验为淫羊藿多糖的临床开发应用提供了依据。

To optimize the extraction technology of Epimedium polysaccharide from Herba Epimedii. The extraction technology was used by using ultrasonic-assisted extraction technology and hot water extraction technology. On the basis of single factor test results, enzyme concentration, enzyme extraction temperature, enzyme extraction time were selected as independent variables. Three factors and three levels of the Box-Behnken response surface design was used to that of optimizing extraction of Epimedium polysaccharide. The results showed that the optimum parameters for extracting Epimedium polysaccharide, enzyme concentration was 110 U/μL, temprature of enzyme extraction is 60 ℃, enzyme extraction time is 10 minutes. The enzyme extraction temperature had the greatest effect on the extraction rate of polysaccharide. Under the optimum conditions, the extraction rate of polysaccharide was 10.06%, which closes to the maximum expected value of extraction（10.199%） of the model. The experiment had provided the basis for the clinical application of Epimedium polysaccharide.