海参西洋参枸杞子口服液对免疫低下小鼠免疫功能的增强作用

Enhancing effects of Haishen-Xiyangshen-Gouqizi Koufuye on the immune function of immunocompromised mice

目的评价海参西洋参枸杞子口服液(HXGK)对免疫低下小鼠免疫功能的调节作用。方法 MTT法测试HXGK对小鼠RAW264.7单核巨噬细胞的毒性,比色法检测RAW264.7细胞吞噬中性红能力。体内实验采用免疫低下小鼠模型(注射地塞米松造模),实验设正常对照组、模型组、阳性药左旋咪唑25 mg/kg组和HXGK 5、10、20 ml/kg给药组;除正常对照组以外,其余各组在分组当日及其后每隔1 d腹腔注射地塞米松40 mg/kg 1次,共注射5次;在首次注射地塞米松后次日开始每日灌胃给药,正常对照组和模型对照组给予生理盐水,共给21 d,其后测定小鼠碳粒廓清指数、脾指数和胸腺指数、外周血白细胞和淋巴细胞数及Ig A和Ig G含量。结果与空白组相比,HXGK在100倍稀释和20倍稀释剂量下将RAW264.7细胞吞噬指数从1.00分别提高到1.12和1.32(均为P<0.01);在免疫力低下小鼠体内实验中,与模型组相比,HXGK 5、10、20 ml/kg剂量组小鼠的吞噬指数分别增强1.8(P<0.01)、1.5和1.7倍(均为P<0.05),脾指数从1.60分别恢复到2.96、2.56(均为P<0.01)和2.32(P<0.05),胸腺指数从1.31分别恢复到1.46、1.59和1.71(均为P<0.05),外周血白细胞数分别提高1.32、1.75和1.46倍(均为P<0.05),淋巴细胞数分别提高约16、20和19倍(均为P<0.01),血中抗体Ig G含量分别提高约19%、57%(P<0.01)和64%(P<0.05),Ig A含量分别提高约65%(P<0.01)、47%(P<0.05)和44%(P<0.01)。结论 HXGK对免疫低下小鼠的免疫功能有显著增强作用。

Objective To evaluate the immunomodulatory effect of Haishen-Xiyangshen-Gouqizi Koufuye（HXGK）,an oral liquid of healthy food product,on the immunocompromised mouse model. Methods The cytotoxicity was assayed by the MTT method using murine monocyte macrophagse Raw264.7 cells. The in vitro phagocytic activity of RAW264.7 cells was assayed by the colorimetric neutral red phagocytosis test. In the in vivo mouse test,animals were randomized into six groups,each with ten mice：the normal control,model control,positive control groups and three HXGK（5,10,and 20 ml/kg）test groups. The immunocompromised mouse model was created by the intraperitoneal injection of 40 mg/kg dexamethasone once every other day for a total of five times. After first time injection of dexamethasone,the normal control（without the dexamethasone injection）and model control groups were administered orally once a day with saline,the positive control group with 25 mg/kg levamisole,and the three test groups with 5,10 and 20ml/kg HXGK,respectively,for a total of 21 days. Then the carbon particle clearance index,the spleen and thymus indices,and the leukocytes,lymphocytes,Ig G and Ig A in peripheral blood were measured respectively. Results Compared with the normal control,HXGK significantly enhanced the phagocytic index of RAW264.7 cells from 1.00 to 1.12（P〈0.01）and 1.32（P〈0.01）at the 100-and 20-fold diluted dosages,respectively,in the in vitro neutral red phagocytosis test. In the in vivo mouse test,compared with the model control group,HXGK at the doses of 5,10 and 20 ml/kg obviously increased the carbon particle clearance index about 1.8（P〈0.01）,1.5（P〈0.05）and 1.7-fold（P〈0.05）and improved the spleen index from 1.60 to 2.96（P〈0.01）,2.56（P〈0.01）and 2.32（P〈0.05）,the thymus index from 1.31 to 1.46,1.59（P〈0.05）and 1.71（P〈0.05）,respectively. Meanwhile,HXGH at the 5,10 and 20 ml/kg dosages also increased the leukocytes about 1.32,1.75（P〈0.05）and 1.46 folds（P〈0.05）,the lymphocytes about 16（P〈0.01）,20（P〈0.01）and 19 folds（P〈0.01）,the Ig G level about 19%,57%（P〈0.01）and 64%（P〈0.05）,and the Ig A level about 65%（P〈0.01）,47%（P〈0.05）and 44%（P〈0.01）,all in the peripheral blood respectively. Conclusion HXGH could significantly enhance the immune function of the immunocompromised mice.