桂附地黄丸中多糖指纹图谱的建立

Establishment of fingerprint profiles of polysaccharides extracted from Guifu Dihuang Wan

目的以桂附地黄丸中多糖为研究对象,初步探索中药多糖质量控制的新方法。方法对桂附地黄丸多糖部位进行控制条件下的部分水解,HPLC检测水解产物,得到指纹图谱。以中药指纹图谱评价软件和Microsoft Excel 2002图表处理图谱信息。结果在不同来源共10个批号的桂附地黄丸多糖部位指纹图谱中,8个批号的峰面积相似度>0.99,2个批号相似性分别为0.92和0.94。结论初步确定可采用"多糖水解-HPLC指纹图谱"的方法对桂附地黄丸中的多糖进行质量控制。

Objective To find a new method for quality control of polysaccharides by establishing the fingerprint profiles of polysaccharides extracted from Guifu Dihuang Wan. Methods The polysaccharides extracted from Guifu Dihuang Wan were hydrolyzed partially under appropriate hydrolysis condition with acid,and the fingerprint profiles of the hydrolyzates was obtained by HPLC.Microsoft Excel 2002 was applied for calculating the similarity of fingerprint profiles,and informatton analysis of these profiles was performed by a software for evaluating fingerprint profiles of traditional Chinese medicine. Results Fingerprint profiles of the hydrolyzates of polysaccharides from 10 of Guifu Dihuang Wan products,produced by different manufacturers,were obtained. It was observed that the similarity of eight products was greater than 0.99,while the similarity of the other two was 0.92 and 0.94,respectively. Conclusion This method is technically feasible for the quality control of polysaccharides from Guifu Dihuang Wan.