摘要
作者用PAP免疫组化、显微分光光度计扫描、微机统计处理等方法,发现在常压缺氧情况下,大鼠心肌内肌红蛋白呈灶状缺失,并随缺氧时间的延长,缺失灶数目增多,范围扩大。结果提示,肌红蛋白缺失灶是单纯性缺氧时心肌的早期改变。为研究缺氧时心肌细胞受损的早期改变及可复性,提供了较为敏感而可靠的检测方法。
The change of myocardial myoglobin content induced by normobaric hypoxia in rats was studied with peroxidase—anti—peroxidase(PAP)method as well as scanning microspectrophotometry. It was found that under the condition of normobaric hypoxia, myocardial myoglobin of rats was focally lost. The size and number of myoglobin loss foci were positively related to the duration of hypoxia. These results indicate that focal loss of myocardial myoglobin appears to be the early manifestation of myocardial damage caused by hypoxia. This study also provides a sensitive and reliable method for detecting early damage of myocardium during hypoxia and its reversibility.
出处
《中国循环杂志》
CSCD
1991年第6期579-581,共3页
Chinese Circulation Journal
关键词
缺氧
肌红蛋白
免疫组织化学
心肌
Myocardial myoglobin
Hypoxia
Immunohistochemistry