转移抑素抑制人鼻咽癌高转移能力细胞株SUNE-1-5-8F转移侵袭能力的研究

A study of metastin on inhibiting the capacity of metastasis and invasion in highly metastatic nasopharyngeal carcinoma SUNE-1-5-8F cells

目的研究并探讨转移抑素(metastin)对人鼻咽癌高转移能力细胞株SUNE-1-5-8F转移侵袭能力的影响。方法 (1)采用Transwell体外侵袭趋化实验技术,在不同浓度(10~2nM、10~3nM、10~4nM)转移抑素干预前后计数转移及侵袭细胞数,探究其对SUNE-1-5-8F细胞转移能力和侵袭能力的影响。(2)采用BALB/c-nu裸鼠肺转移模型,40只裸鼠随机平均分为转移抑素组及对照组,2组均右侧腋窝下注射2×10~6/ml SUNE-1-5-8F细胞悬液,其中转移抑素组于第2天按1.17 mg/kg腹腔注射转移抑素,研究其对裸鼠移植瘤的抑瘤率、转移抑制率和生命延长率的影响;采用免疫组化法研究肺转移灶kisspeptin指标表达情况。结果 (1)Transwell实验中转移抑素对于SUNE-1-5-8F细胞的转移能力及侵袭能力均具有抑制作用(P<0.05),且其抑制能力与转移抑素浓度呈正相关(P<0.05)。(2)裸鼠实验中转移抑素对抑瘤率无明显影响(P>0.05),但可以降低肺转移数,提升转移抑制率(P<0.05),且进一步提高了小鼠的生命延长率(P<0.05)。免疫组化示转移抑素组kisspeptin较对照组高表达。结论 (1)Transwel实验中转移抑素能够抑制人鼻咽癌高转移能力细胞株SUNE-1-5-8F的转移侵袭能力,且转移抑素浓度与其抑制能力呈正相关。(2)裸鼠实验中转移抑素能够降低肺转移率,延长裸鼠生命,但与腋下移植瘤的增殖无显著相关性。

Abstract：Objective To investigate and discuss the effect of metastin on inhibiting the capacity of metastasis and inva- sion in highly metastatic nasopharyngeal carcinoma SUNE-1-5-8F cells. Methods （1）The SUNE-1-5-8F cells＇ were trea- ted with different concentration of metastin（ 102 nM, 103 nM, 104 nM）. Contrast the cells before and after treatment. Final- ly, the capacity of metastasis and invasion were tested by transwell assay. （2）Forty female mouse were randomly dividend into metastin group and control group. Both were injected with 2× 106/ml. SUNE-1-5-8F cells under the right armpits. Mouse in metastin group were injected with 1.17 mg/kg metastin at day 2. The tumor inhibition rate, metastasis inhibition rate and life extension rate were detected by BALB/c-nu nude mouse model assay. The expression of kisspeptin protein was analyzed by immunohistochemistry. Results （1）Metastin inhibited the SUNE-1-5-8F cells＇ capacity of metastasis and invasion in cell assay（ P 〈 0.05 ）, whose capacity had a positive correlation with the concentration of metastin. （2）Metastin didn＇ t inhibit the proliferation of tumor in nude mouse assay （ P 〉 0.05 ） , but it reduced the pulmonary metastasis （ P 〈 0.05 ）. And metastin influenced the life extension rate of nude mouse （ P 〈 0.05 ）. The result of immunohistochemistry showed that the expression of kisspeptin protein of the metastin group was significantly different from the control group （P 〈 0.05 ）. Conclusions （1）Metastin inhibits the SUNE-1-5-8F cells＇ capacity of metastasis and invasion in cell assay, whose capacity has a positive correlation with the concentration of metastin. （2）Metastin reduces the pulmonary metas- tasis and prolonged life, but it doesn＇ t inhibit the proliferation of tumor in nude mouse assay.