Box-Behnken法优化加酸超声提取瞿麦中大黄素的工艺

Optimization of ultrasound extraction technology with acid of emodin from herba dianthi by response surface methodology

采用响应面法优化瞿麦中大黄素的提取工艺.利用单因素法进行考察,以大黄素提取量为指标,考察液固比、超声时间、温度、盐酸质量分数及用量对大黄素提取量的影响,并结合Design-Expert软件中的Box-Behnken实验设计和响应面分析方法对超声提取瞿麦中大黄素的工艺进行优化,再与药典中虎杖提取大黄素方法进行比较,确定大黄素超声提取最佳工艺:液固比为61倍,温度为40℃,超声30 min,加质量分数为8%的盐酸1 m L.通过优化得到的大黄素超声提取工艺省时,稳定,且与回流法相比提取量高.

Response surface methodology was used to optimize the optimal ultrasound extraction technology of emodin from herba dianthi. With the amount of extraction of emodin as an indicator, single-factor test was used to investigate the effect of ethanol volume fraction, solid-liquid ratio, ultrasound time, temperature, mass fraction of hydrochloric acid and dosage on the amount of extraction of emodin. The re- sults were combined with Box-Benhken design of design-expert software and response surface analysis to optimize the technology parameters for extraction process of herba dianthi, and compare with extraction technology of emodin from Polygonum cuspidatum in the Pharmacopoeia. Obtain the optimal ultrasound extraction technology under the following conditions, liquidsolid ratio was 61, 40 ~C, 30 rain, hydro- chloric acid mass fraction 8%, 1 mL. Ultrasound extraction technology of emodin by response surface methodology is time-saving, stable, and the amount of extraction is higher than condensation reflux method. It＇s provide theoretical basis for quality evaluation of Dianthus superbus.