摘要
目的分析广西壮族人群白细胞介素(IL)-27基因rs17855750G/T、rs40837A/G多态性分布特点,对比其基因型和等位基因频率与不同国家(地区)间种族的分布差异。方法采用单碱基延伸的PCR技术对168例广西壮族人群IL-27基因rs17855750G/T、rs40837A/G进行多态性检测,对比国际人类基因组计划(HapMap)公布的中国北京人、日本人、非洲人和意大利人的单核苷酸多态性(SNP)分型数据,分析5个人群rs17855750G/T、rs40837A/G位点的基因型和等位基因频率差异。结果在广西壮族人群中IL-27基因rs17855750 G/T位点TT基因型最常见,约为70.2%,T等位基因频率最高,约为50.3%,rs40837A/G多态性位点AA基因型最常见,约为35.7%,A等位基因频率最高,约为52.1%,IL-27基因rs17855750 G/T、rs40837A/G多态性位点基因型及等位基因频率男女性别间比较差异无统计学意义(P>0.05),IL-27rs17855750G/T与北京人比较差异无统计学意义(P>0.05),但与日本人、意大利人和非洲人比较差异有统计学意义(P<0.01);IL-27rs40837A/G与北京人比较差异有统计学意义(P<0.05),且与日本人、非洲人和意大利人比较差异也有统计学意义(P<0.01)。结论 IL-27基因rs17855750G/T、IL-27rs40837A/G位点基因型和等位基因在广西壮族人群中的分布频率与其他国家(地区)人群相比存在差异,这种差异可能是导致某些疾病在不同人群发病率和临床表现存在差异的原因之一。
Objective To investigate the distribution characteristics of interleukin-27 gene rs17855750 G/T, rs40837 A/G polymorphism in Zhuang populations of Guangxi,and to compare the distribution differences of genotype and allele frequencies of interleukin-27 gene rs17855750 G/T, rs40837 A/G polymorphisms among different races. Methods The interleukin-27 gene rs17855750 G/T,rs40837 A/G polymorphisms, were detected by SNaPshot SNP genotyping technique on 168 persons in Zhuang populations of Guangxi, frequencies of genotype and allele of interleukin-27 gene rs17855750 G/T, rs40837 A/G polymorphisms were analyzed in Zhuang populations,and was compared with the other four populations (HapMap-HCB, HapMap-JPT, HapMap- YRI,HapMap-TSI) from HapMap database. Results The most common genotype and allele of interleukin-27 gene rs17855750 G/ T polymorphysms were TT(70.2%) and G(50.3%) in Zhuang populations of Guangxi,and the most common genotype and allele of interleukin-27 gene rs40837 G/T polymorphysms were AC(35.7 %) and C(52.1 %). There were no significant differences in the genotype and allele frequencies of interleukin-'27 gene rs17855750 G/T, rs40837 A/G polymorphysms between male and female gender in Zhuang populations of Guangxi(P〈0. 05). The frequencies of allele and genotype distribution of IL-27 gene rs17855750 G/T polymorphisms were not significantly different when compared with HapMap-HCB(P〈0.05), but were significantly different when compared with HapMap-JPT, HapMap-TSI and HapMap-YRI(P〈0.01) ,The frequencies of allele and genotype distribution of in- terleukin-27 gene rs40837 A/G polymorphisms were significantly different when compared with HapMap-HCB(P〈 0. 05), and were significantly different when compared with HapMap-JPT,HapMap-YRI and HapMap-TSI(P〈0.01). Conclusion There are significant differences in the frequencies of allele and genotype distribution of interleukin-27 gene rs17855750 G/T, rs40837 A/G between Zhuang populations and other ethnic populations,and this variation may lead to a variety of clinical manifestation and morbidity of some diseases.
作者
胡仁统
王俊利
韦叶生
陆潞
韦贵将
黄可
廖品琥
唐毓金
Hu Rentong Wang Junli Wei Yesheng Lu Lu Wei Guijiang Huang Ke Liao Pinhu Tang Yujin(Affiliated Hospital of Youjiang Medical University of Nationalities ,Baise,Guaqngxi 533000,Chin)
出处
《重庆医学》
CAS
北大核心
2017年第23期3169-3172,3176,共5页
Chongqing medicine
基金
国家自然科学基金资助项目(81260315
81541136)
广西自然科学基金资助项目(2012GXNSFAA053169)
广西卫生厅自筹经费科研课题项目(Z2014520)
百色科技攻关项目(20160610)
广西高校青年教师基础能力提升项目(KY2016YB347)