槲皮素对TNF-α诱导的乳鼠心肌成纤维细胞增殖及P38MAPK、HMGB1蛋白表达的影响

Effects of quercetin on proliferation and expression of P38MAPK and HMGB1 protein in neonatal rat cardiac fibroblasts induced by TNF-α

目的研究槲皮素对肿瘤坏死因子α(TNF-α)诱导的心肌成纤维细胞增殖及P38MAPK、高迁移率族蛋白B1(HMGB1)表达的影响。方法通过胰酶消化法和差速贴壁法获得纯化的心肌成纤维细胞,应用四甲基偶氮唑蓝(MTT)法检测心肌成纤维细胞的增殖,采用Western blot法测定P38MAPK、HMGB1蛋白的表达。结果槲皮素对基础状态下的心肌成纤维细胞的增殖无影响,但对TNF-α诱导的心肌成纤维细胞的增殖有抑制作用,且各组细胞的吸光度(A)值随槲皮素浓度的增加而减小(P<0.05),各组细胞P38MAPK、HMGB1蛋白表达随槲皮素浓度的增加而减弱(P<0.05)。结论槲皮素对TNF-α诱导的心肌成纤维细胞增殖有一定地抑制作用,其机制可能是通过P38MAPK信号通路抑制HMGB1的表达,参与抑制心肌成纤维细胞的增殖。

Objective To study the effect of quercetin on proliferation and expression of P38MAPK and HMGB1 protein in neonatal rat cardiac fibroblasts induced by TNF-α. Methods Purified cardiac fibroblasts were obtained by trypsin digestion and differential adherence method. The proliferation of cardiac fibroblasts was detected by MTT assay. The expression of P38MAPK and HMGB1 protein was detected by Western blot. Results Quercetin had no effect on the proliferation of cardiac fibroblasts in the basal state but inhibited the proliferation of cardiac fibroblasts induced by TNF-α,and the A value of each group was increased with the increase of quercetin concentration（P〈0.05）. The exspression of P38MAPK .HMGB1 were decresed with the icrease of quereetin. Conclusion Qu.ereetin may inhibit the proliferation of cardiac fihroblasts induced by TNF-α. The mechanism may be inhibit the expression of HMGB1 through P38MAPK signaling pathway.