乳腺癌术后患者三氧治疗对血清TK1水平变化的影响

Changes of serum TK1 level in breast cancer patients with resection after ozone therapy

目的:探讨切除术后乳腺癌患者三氧治疗前后血清胸苷激酶1(thymidine kinase-1,TK1)水平的变化及其与肿瘤分期的关系。方法:应用酶免疫点印记化学发光法检测104例切除术后乳腺癌患者三氧疗法前后血清TK1水平。比较不同临床分期乳腺癌患者接受三氧疗法前后TK1、CA15-3及CEA水平的差异,分析TK1与雌激素受体(ER)和孕激素受体(PR)的关系。结果:三氧治疗前乳腺癌患者TK1平均值为(1.52±1.53)pmol/L,与健康体检组的(0.54±0.42)pmol/L相比,差异有统计学意义(P=0.000);三氧治疗后TK1平均值为(0.73±0.82)pmol/L,与治疗前相比,差异有统计学意义(P=0.001)。根据临床分期,Ⅰ-Ⅲ期患者治疗前TK1平均值为(1.05±1.17)pmol/L,治疗后平均值为(0.38±0.44)pmol/L,差异有统计学意义(P=0.002);Ⅳ期患者治疗前TK1平均值为(2.53±1.73)pmol/L,治疗后平均值为(1.47±0.94)pmol/L,差异无统计学意义(P=0.080)。CA15-3及CEA在三氧治疗前后比较,差异均无统计学意义,但Ⅰ-Ⅲ期患者CA15-3及CEA与TK1变化趋势有一致性。TK1与ER、PR有关联(r=0.455,P=0.000)。结论:三氧治疗能有效降低切除术后乳腺癌患者的TK1水平,抑制肿瘤细胞增殖,控制疾病进展,且对Ⅰ-Ⅲ期患者更有意义。

Objective： To investigate the changes of serum TK1 levels in patients of breast cancer with resection after ozone therapy,and explore the relationship between the changes of TK1 and tumor stage. Methods： Serum TK1 levels were detected in 104 breast cancer patients with resection before and after ozone therapy by enzyme immunoassay dot blot chemiluminesence assay. To compare the difference of TK1、CA15-3 and CEA in breast cancer patients with different clinical stages before and after three oxygen therapy,and to analyze the relationship between TK1 and estrogen receptor（ ER） and progesterone receptor（ PR）. Results： The average TK1 in breast cancer patients before three oxygen therapy was（ 1. 52 ± 1. 53） pmol/L,compared with the healthy group（ 0. 54 ± 0. 42） pmol/L,the difference was statistically significant（ P = 0. 000）. The mean value of TK1 after three oxygen treatment was（ 0. 73 ±0. 82） pmol/L,compared with before treatment,the difference was statistically significant（ P = 0. 001）. According to clinical stage,the average TK1 in stage Ⅰ-Ⅲ patients before treatment was（ 1. 05 ± 1. 17） pmol/L,after treatment average was（ 0. 38 ± 0. 44） pmol/L,the difference was statistically significant（ P = 0. 002）. The average TK1 in stage IV patients before treatment was（ 2. 53 ± 1. 73） pmol/L,after treatment average was（ 1. 47 ± 0. 94） pmol/L,the difference was not statistically significant（ P = 0. 080）. There was no significant difference in CA15-3 and CEA before and after three oxygen treatment（ P〉0.05）,but the CA15-3 and CEA in stage Ⅰ-Ⅲ patients had consistency with TK1 change trend. The level of TK1 was related to PR and ER（ r = 0. 455,P = 0. 000）. Conclusion： Ozone therapy can effectively reduce the TK1 level of breast cancer patients after resection,inhibit the proliferation of tumor cells,control the disease progression,and it is more significant for patients with stage Ⅰto Ⅲ.