白藜芦醇抑制人肝癌SMMC-7721细胞增殖并降低mTOR蛋白磷酸化水平

Effect of resveratrol on proliferation of liver cancer SMMC-7721 cells and lowering levels of mTOR protein phosphorylation

目的将不同浓度白藜芦醇作用于人肝癌SMMC-7721细胞,研究中药提取物对肝癌的抑癌机制。方法使用不同浓度的白藜芦醇作用于人SMMC-7721细胞,生长曲线和克隆形成实验检测白藜芦醇对SMMC-7721细胞的影响,流式细胞术检测白藜芦醇对SMMC-7721细胞细胞周期的作用,Westren blot方法检测mTOR及其磷酸化水平变化。结果与对照组比较,白藜芦醇组可抑制SMMC-7721细胞增殖,呈量效依赖关系,且差异有统计学意义(P<0.01);细胞周期检测显示白藜芦醇可阻滞SMMC-7721细胞于G_0/G_1期;Westren blot提示白藜芦醇可降低mTOR磷酸化水平。结论白藜芦醇在一定浓度范围内可抑制人SMMC-7721细胞增殖,阻滞细胞周期于G_0/G_1期,其抑制机制可能与白藜芦醇降低mTOR磷酸化水平有关。

Aim To study the effect of resveratrol on proliferation of liver cancer SMMC-7721 cells and the relevant mechanism. Methods SMMC-7721 cells were treated with different concentrations of resveratrol. Cell proliferation was tested by cell proliferation assay and colony-forming experiment. The effect of resveratrol on cell cycle of SMMC-7721 cells was detected by flow cytometry. The activation of mTOR/p-mTOR proteins was detected by Western blot. Results Compared with control group,resveratrol inhibited SMMC-7721 cell proliferation in a dose-dependent manner（ P 0. 01）; cell cycle detection showed that SMMC-7721 cells were arrested at G0/G1 phase by resveratrol;Westren blot assay showed that resveratrol down-regulated p-mTOR expression. Conslusion Resveratrol inhibits human SMMC-7721 cell proliferation and arrests the cell cycle at G0/G1 phase,while the inhibition mechanism may be related to activation of mTOR signaling pathway by resveratrol.