精料水平和复合抗菌肽对川中黑山羊生长性能及血清中免疫球蛋白、补体、细胞因子和激素水平的影响

Effects of concentrate level and dietary supplementation of recombinant antimicrobial peptides(AMPs) on growth performance and serum immunoglobulin,cytokines,complement,hormone levels in male Chuanzhong black goats

试验旨在研究精料水平和添加复合抗菌肽对川中黑山羊生长性能及免疫球蛋白、补体、细胞因子、激素水平的影响。将24只4月龄川中黑山羊按照体质量相近的原则分为4组,每组6只。Ⅰ组为普通精料对照组(300 g精料),Ⅱ组为普通精料+抗菌肽组(300 g精料+3.0 g抗菌肽),Ⅲ组为高精料组(600 g精料),IV组为高精料+抗菌肽组(600 g精料+3.0 g抗菌肽)。预饲1周后开始试验,试验期60 d。于0、20、40、60d时,每组选取4只山羊颈静脉采血制备血清,检测免疫球蛋白、补体、细胞因子和激素指标,同时每组每只山羊依次空腹称量体质量。结果显示:与普通精料对照组相比,添加复合抗菌肽和饲喂高精料组的山羊血清中免疫球蛋白IgA、IgG、IgM,细胞因子IL-2、IL-6、IL-10、IL-12、IFN-γ、TNF-α,补体C3、C4,生长激素ACTH、IGF-1、T3、T4等指标有明显升高的趋势,且山羊体质量和平均日增重均有显著增加。证实复合抗菌肽以及高精料均能够增强山羊机体的免疫功能,促进生长。

This study is to evaluate the effects of dietary additive of antimicrobial peptides（AMPs） on growth performance,immunoglobulin,cytokines,complement and hormone levels of male Chuanzhong black goats.A total of 24 male goats were allotted to four groups on the basis of initial body weight,each group with 6 goats.Dietary treatments were Ⅰ-normal concentrate group（300 g concentrate）,Ⅱ-normal concentrate and antimicrobial peptide group（300 g concentrate ＋ 3.0 g antimicrobial peptides）,Ⅲ-high concentrate group（600 g concentrates）,Ⅳ-high concentrate and antimicrobial peptide group（600 g concentrate ＋ 3.0g antimicrobial peptides）.After a week of pre-feeding test,the 60 day test was conducted.On 0,20th,40th and 60th day,4 goats were randomly selected from each group,whose jugular vein blood samples were collected to test immunoglobulin,cytokines,complement and hormone levels,and were weighed one by one.The results showed,compared with the control group,the recombinant antimicrobial peptides and high concentrate could significantly increase the levels of immunoglobulin IgA,IgG,IgM,cytokine IL-2,IL-6,IL-10,IL-12,IFN-γ,TNF-α,complement C3,C4,hormone ACTH,IGF-1,T3,T4 in serum,the body weights and average daily gains,which confirmed that the recombinant antimicrobial peptides and high concentrate could enhance the immune function and promote the growth of the goat.