脑胶质瘤中RAD18的表达与放射治疗抵抗相关性分析

Correlation between RAD18 expression and radiotherapy resistance in glioblastoma multiforme

目的探讨RAD18与多形性胶质母细胞瘤(Glioblastoma multiforme,GBM)放射抵抗的相关性,以期为改善脑胶质瘤放射抵抗提供新的治疗靶点。方法将人脑胶质瘤细胞系A172分别转染空白和装有RAD18的质粒载体,应用克隆实验检测经相同剂量射线照射后两组细胞增殖情况,最后采用qRT-PCR方法检测原发性及放射性粒子近距离治疗后复发性GBM样本中RAD18 mRNA的表达情况,进行统计学分析。结果经转染RAD18质粒过表达的GBM细胞经放射治疗后增殖情况高于转染空白质粒的GBM细胞(P<0.001);经近距离放射性粒子治疗后复发性GBM中RAD18 mRNA的表达水平高于原发性GBM(P<0.01)。结论复发性GBM放射治疗的抵抗性可能与RAD18的蛋白过表达有关。

Objective The objective of this study was to investigate the relationship between the expression of RAD18 and radiation resistance in glioblastoma multiforme（GBM）and to provide a new therapeutic target for improving the radiation resistance of GBM.Methods Human glioma A172 cells were transfected into blank and RAD18-containing plasmid vector.The cell proliferation of two groups after the same dose radiation was detected by cloning assay.The mRNA expression of RAD18 in primary and recurrent GBM samples after close proximity treatment were detected by qRT-PCR.All data were analyzed statistically.Results The proliferation of GBM cells transfected with RAD18 plasmid was higher than that of cells transfected with blank plasmid after radiation therapy（P〈0.001）.The expression level of RAD18 mRNA in recurrent GBM was higher than that in the untreated radioactive granules primary GBM（P〈0.01）.Conclusion The resistance of recurrent GBM to radiotherapy may be associated with the overexpression of RAD18 protein.