摘要
目的观察过表达miR-409-5p对肝癌细胞系HepG2增殖及迁移能力的影响。方法利用miR-409-5p mimics进行转染肝癌细胞系HepG2,并通过实时荧光定量PCR进行验证。高表达miR-409-5p后,使用MTT法和划痕实验分别检测HepG2细胞增殖能力及迁移能力的变化。结果MTT实验显示,HepG2细胞转染miR-409-5p mimics后细胞增长趋势减缓,且随miR-409-5p mimics浓度和时间成正比。划痕实验显示,在24 h和48 h两个时间点三组间比较差异均有统计学意义(P<0.05),证实过表达miR-409-5p mimics的细胞体外迁移能力明显低于转染阴性对照组(NC mimics)组和对照组。结论上调miR-409-5p可抑制肝癌细胞系HepG2的增殖及迁移能力。
Objective The objective of this study was to observe effects of miR-409-5p on the proliferation and migration of HepG2 cells.Methods HepG2 cells were transfected with miR-409-5p mimics and verified by Real-Time quantitative PCR.After high expression of miR-409-5p,MTT and wound healing assays were used to detect the proliferation and migration ability of HepG2 cells.Results The cell viability of HepG2 cells transfected with miR-409-5p mimics was significantly decreased in comparison with the control group and showed a dose-and time-dependent manner（P〈0.05）.The migration ability of cells overexpressing miR-409-5p mimics was significantly lower than that in the NC group（P〈0.05）.There was significant difference between the two groups at treatments for 24 h and 48 h.Conclusion Up-regulated miR-409-5p can inhibit the proliferation and migration of HepG2 cells.
出处
《实用肿瘤学杂志》
CAS
2017年第4期305-309,共5页
Practical Oncology Journal
基金
浙江省院所专项(No.2015D3006)
浙江省中医药科技计划项目(No.2015ZA161)
