土壤杆菌固氮、溶磷活性及对植物的促生作用

Nitrogen fixation,phosphate solubilizing activity and plant growth promotion of Agrobacterium

通过选择性培养基,定性和定量检测了26株土壤杆菌的固氮和溶磷活性,通过盆栽检测了澳洲生防菌株K1026对黄瓜和小麦的促生长作用。结果显示,包括致病性和无致病性的26株土壤杆菌均可在无氮培养基上正常生长;其中19株具有溶磷活性,K1026对磷酸钙液体培养基的溶磷量为109.11μg/m L;对黄瓜幼苗根系生长具有显著促进作用,但对小麦生长没有明显影响。

Through selective medium,the function of fixing nitrogen and dissolving phosphorus of 26 Agrobacterium strains were studied by both qualitative and quantitative analysis methods. Meanwhile,growth-promoting effect of Australian biocontrol strain K1026 on cucumber and wheat was measured by pot experiment. The results showed that all the strains regardless of pathogenicity or non-pathogenicity could grow normally on nitrogen-free medium. 19 of the 26 strains had the ability to dissolve phosphorus,the Phosphate released by K1026 in Pikovskaya broth medium was up to 109. 11 μg/m L,having the strongest activities among all stains. The pot experiments showed that K1026 had obvious growth-promoting effect on the cucumber seedlings,and the root length and the dry weight of the root biomass was increased by 25. 49%,62. 96%,respectively,compared with the control. However,K1026 had no obvious effect on the growth-promotion of wheat.