LSCI检测不同缺血期对大鼠缺血再灌注过程脑血流的影响

Effects of different ischemic duration on post-ischemic and reperfusion rat cerebral blood flow detected by LSCI

目的考察不同缺血期对大鼠缺血再灌注过程脑血流的影响。方法通过激光散斑成像技术(LSCI)检测大鼠大脑中动脉不同栓塞时间后,对再灌注过程中脑血流的影响,并对再灌注过程中的低灌注状态、无复流现象、血流流速及管径变化进行研究分析。结果 2 h的栓塞后,大鼠脑部血流量趋向低灌注状态,约为栓塞前血流基值的(35±10)%;栓塞时间越长,无复流现象越多;在再灌注期间,缺血2 h组的血流比0.5 h组的低灌注水平低10%。结论长时间的脑缺血期再灌注过程可能是造成脑损伤程度加重的主要原因之一。LSCI可应用于脑部血流实时监测,操作简便,成像灵敏、稳定,结果可靠。

Objective To investigate the effects of different ischemic duration on post-ischemic and reperfusion rat cerebral blood flow. Methods Laser speckle imaging system （LSCI） was used to detect the effects of different ischemic duration on post-ischemia and reperfusion rat cerebral blood flow. At the same time, the dynamic changes of capillary flow and diameter, no reflow phenomenon and hypoperfusion were analyzed. Results During 2h middle cerebral artery occlusion （MCAO） ischemia, perfusion of cerebral blood flow decreased to （35±10）% of the baseline, reflecting a state of hypoperfusion. With the prolongation of ischemic time, no reflow phenomenon was aggravated. Hypoperfusion in 2 h group was approximately 10% less than that in 30 min group during the period of ischemia and reperfusion. Conclusion The long ischemic duration on post-ischemic and reperfusion may be one of the main reasons for the increase of the degree of brain injury.The established LSCI method can be applied to real-time monitoring of MCAO rat cerebral blood flow, which is easy to operate, sensitive, stable and reliable.